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PMID: 2997039 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Plasmid-mediated virulence in Salmonella dublin demonstrated by use of a Tn5-oriT construct.

Infection and immunity ·Vol. 50 ·No. 2 ·1985-11-00 ·Pages 420-4

Chikami GK, Fierer J, Guiney DG

Abstract

Salmonella dublin, a serotype which causes invasive disease in cattle and humans, carries a characteristic 80-kilobase plasmid (pSDL2). We were able to cure the plasmid from a strain of S. dublin. The cured strain was avirulent for mice by either the oral or intraperitoneal route of infection. A derivative of Tn5 which contains the transfer origin of the broad-host-range plasmid RK2 (Tn5-oriT) was transposed onto pSDL2, allowing mobilization of the plasmid by an RK2 helper plasmid. Reintroduction of the pSDL2 derivative plasmid into the cured strain restored virulence, demonstrating that the plasmid is necessary for virulence. These studies also demonstrate the usefulness of the Tn5-oriT construct for genetic manipulations.

MeSH Terms
Animals Conjugation, Genetic DNA Restriction Enzymes DNA Transposable Elements Mice Mice, Inbred Strains Plasmids Salmonella/genetics,pathogenicity Salmonella Infections/microbiology Transformation, Bacterial Virulence
Chemicals
DNA Transposable Elements DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Chikami G K
Fierer J
Guiney D G
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28 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.50.2.420-424.1985
Published
1985-11-00
Pages
420-4
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC261968
Subset
IM
Grants
NIAID NIH HHS · AI 16463 · United States
NIADDK NIH HHS · AM 35108 · United States
NIGMS NIH HHS · GM 28924 · United States
External Links
PubMed source DOI: 10.1128/iai.50.2.420-424.1985 PMC Full Text View journal details
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