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PMID: 6090433 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Conjugal transfer of bacterial chromosomes mediated by the RK2 plasmid transfer origin cloned into transposon Tn5.

Journal of bacteriology ·Vol. 160 ·No. 1 ·1984-10-00 ·Pages 451-3

Yakobson EA, Guiney DG

Abstract

We report here a novel system for the conjugal transfer of bacterial chromosomes which utilizes the transfer origin (oriT) of plasmid RK2 cloned into transposon Tn5. Tn5 with oriT was inserted by transposition into the chromosomes of Escherichia coli and Rhizobium meliloti. The oriT sequence then served as the origin of high-frequency chromosome transfer when a helper RK2 plasmid was present in the same cell. The broad host range features of RK2 make this system of oriented chromosome mobilization applicable to most gram-negative bacteria.

MeSH Terms
Anti-Bacterial Agents/pharmacology Chromosomes, Bacterial/physiology Cloning, Molecular Conjugation, Genetic DNA Restriction Enzymes DNA Transposable Elements Drug Resistance, Microbial Escherichia coli/genetics R Factors Rhizobium/genetics
Chemicals
Anti-Bacterial Agents DNA Transposable Elements DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Yakobson E A
Guiney D G
References (20)
20 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.160.1.451-453.1984
Published
1984-10-00
Pages
451-3
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC214743
Subset
IM
Grants
NIGMS NIH HHS · GM 28924 · United States
External Links
PubMed source DOI: 10.1128/jb.160.1.451-453.1984 PMC Full Text View journal details
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