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PMID: 6304722 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Location and nucleotide sequence of the transfer origin of the broad host range plasmid RK2.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 12 ·1983-06-00 ·Pages 3595-8

Guiney DG, Yakobson E

Abstract

The origin of plasmid DNA transfer, oriT, has been localized on RK2, a conjugative drug-resistance plasmid of the IncP group with a very broad host range in gram-negative bacteria. The transfer origin is contained in a 760-base-pair Hae II restriction fragment that maps in the same region as the single-strand nick made by the RK2 relaxation complex. The functional oriT was subcloned as a 112-base-pair Hpa II fragment, and the DNA sequence of this region was determined. The dominant structural feature of the oriT sequence is a 19-base-pair inverted repeat, with 15 of the 19 bases able to form pairs in a hairpin structure. This inverted repeat may be the recognition site for the relaxation complex proteins, which nick the plasmid DNA molecule and initiate the transfer process.

MeSH Terms
Base Sequence Crosses, Genetic DNA Restriction Enzymes DNA, Recombinant/metabolism Escherichia coli/genetics Nucleic Acid Conformation Plasmids
Chemicals
DNA, Recombinant DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Guiney D G
Yakobson E
References (29)
29 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.12.3595
Published
1983-06-00
Pages
3595-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC394096
Subset
IM
Grants
NIGMS NIH HHS · GM 28924 · United States
Databases
GENBANK
K00832
External Links
PubMed source DOI: 10.1073/pnas.80.12.3595 PMC Full Text View journal details
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