Plasmid ColE1 conjugal mobility: the nature of bom, a region required in cis for transfer.

Molecular & general genetics : MGG ·Vol. 185 ·No. 2 ·1982-00-00 ·Pages 344-51

Finnegan J, Sherratt D

Abstract

Conjugal mobility of ColE1 and related plasmids is promoted by a wide range of conjugative plasmids. ColE1 produces trans-acting products and has a region required in cis (bom ; basis of mobility) for such mobility. Here we show that plasmid pBR322 contains a functional bom sequence located within a 141 bp HhaI fragment. This bom region is functional for conjugation promoted by several different conjugative plasmids and is highly conserved in ColE1 and contains nic the putative origin of transfer. The orientation and position of bom with respect to the ColE1 vegetative origin of replication can be changed without affecting the frequency of conjugal mobility promoted by R64drd11.

MeSH Terms

Bacteriocin Plasmids Chromosome Mapping Conjugation, Genetic Escherichia coli/genetics Genes, Bacterial Genetic Linkage Plasmids

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Finnegan J

Sherratt D

References (17)

17 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00330810

Published

1982-00-00

Pages

344-51

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00330810

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Plasmid ColE1 conjugal mobility: the nature of bom, a region required in cis for transfer.

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