Proteins synthesized in minicells containing plasmid ColE1 and its mutants.
Minicells containing the ColE1 plasmid and derivatives of it that contain a Tn3 insertion and deletions of the plasmid were shown to synthesize a variety of polypeptides: (i) a 56,000-dalton polypeptide was found that is colicin E1. (ii) A 15,000-dalton polypeptide is involved in plasmid DNA relaxation. (iii) A 14,000-dalton polypeptide may be the colicin immunity protein. (iv) A correlation between the location of various Tn3 insertions and the proteins synthesized from the plasmid DNA was found that suggests the locations of promoter sites in the plasmid and directions of transcription in two regions of the plasmid. (v) Polypeptides associated with the presence of Tn3 were identifiable. (vi) The nature and extent of gene expression of deletion mutants only generally correlates with the extent of the deletions.
21 references, click to expand
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Mitomycin C-induced expression of trpA of Salmonella typhimurium inserted into the plasmid ColE1.J Bacteriol. 1977 Jan;129(1):388-94 PMID: 318646
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Isolation, mapping, and examination of effects of TnA insertions in ColE1 plasmids.J Bacteriol. 1977 Jan;129(1):482-91 PMID: 318651
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The transposon Tn1 as a probe for studying ColE1 structure and function.Mol Gen Genet. 1977 Mar 7;151(2):151-60 PMID: 327263
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Isolation and genetic analysis of deletion mutants of colicin E1 plasmids carrying a TnA insertion.J Bacteriol. 1977 Oct;132(1):321-31 PMID: 334728
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Studies of colicin E1 plasmid functions by analysis of deletions and TnA insertions of the plasmid.J Bacteriol. 1977 Oct;132(1):332-40 PMID: 334729
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Protein expression in E. coli minicells by recombinant plasmids.Cell. 1977 Mar;10(3):521-36 PMID: 403011
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The generation of a ColE1-Apr cloning vehicle which allows detection of inserted DNA.Mol Gen Genet. 1975 Dec 30;142(3):239-49 PMID: 1045010
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Origin of the TEM-beta-lactamase gene found on plasmids.J Bacteriol. 1975 Apr;122(1):250-6 PMID: 1091625
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Relaxation complexes of plasmid DNA and protein. II. Characterization of the proteins associated with the unrelaxed and relaxed complexes of plasmid ColE1.J Biol Chem. 1975 Nov 25;250(22):8790-5 PMID: 1102544
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Derepression of anthranilate synthase in purified minicells of Escherichia coli containing the Col-trp plasmid.J Bacteriol. 1973 Aug;115(2):615-22 PMID: 4579874
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Purification and properties of colicin E3 immunity protein.J Biol Chem. 1974 Jan 25;249(2):438-44 PMID: 4588566
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E3 immunity substance. A protein from e3-colicinogenic cells that accounts for their immunity to colicin E3.J Biol Chem. 1974 Jan 25;249(2):445-53 PMID: 4588567
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Identification of messenger ribonucleic acids and proteins synthesized by the bacteriocinogenic factor Clo DF13 in purified minicells of Escherichia coli.J Bacteriol. 1974 Apr;118(1):213-24 PMID: 4595198
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Transposition of ampicillin resistance from RP4 to other replicons.Mol Gen Genet. 1974;132(1):31-40 PMID: 4609125
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Segregation into and replication of plasmid deoxyribonucleic acid in chromosomeless segregants of Escherichia coli.J Bacteriol. 1970 Jun;102(3):642-7 PMID: 4914069
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Properties of a supercoiled deoxyribonucleic acid-protein relaxation complex and strand specificity of the relaxation event.Biochemistry. 1970 Oct 27;9(22):4428-40 PMID: 4919547
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Synthesis of ribonucleic acid and protein in plasmid-containing minicells of Escherichia coli K-12.J Bacteriol. 1971 Jul;107(1):21-33 PMID: 4935321
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Physical and functional characteristics of R-factor deoxyribonucleic acid segregated into Escherichia coli minicells.J Bacteriol. 1971 Oct;108(1):300-8 PMID: 4941560
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Purification and characterization of colicin E1.J Biol Chem. 1971 Oct 25;246(20):6318-27 PMID: 5001789
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Cleavage of structural proteins during the assembly of the head of bacteriophage T4.Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
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