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PMID: 6360905 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A small plasmid in Shigella dysenteriae 1 specifies one or more functions essential for O antigen production and bacterial virulence.

Infection and immunity ·Vol. 43 ·No. 1 ·1984-01-00 ·Pages 391-6

Watanabe H, Timmis KN

Abstract

The role of plasmids in the virulence of Shigella dysenteriae 1 W30864, which contains at least five species, was investigated. By means of a standard plasmid-curing procedure, that is, bacterial cultivation at an elevated temperature, five virulence-deficient derivatives were obtained. One of these lacked a small, 6-megadalton plasmid, designated pHW400, exhibited reduced invasiveness for HeLa cells, and failed to produce the somatic antigen. Transposon tagging of the pHW400 plasmid to produce pHW401 and the transfer of this derivative into a variant of strain W30864 lacking pHW400 confirmed the conclusion that the pHW400 plasmid encodes one or more functions involved in O antigen (lipopolysaccharide) biosynthesis and bacterial virulence. A plasmid of similar size was detected in all of the three other strains of S. dysenteriae 1 examined.

MeSH Terms
Antigens, Bacterial/genetics HeLa Cells/microbiology Humans Phenotype Plasmids Serotyping Shigella dysenteriae/immunology,pathogenicity Virulence
Chemicals
Antigens, Bacterial
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Watanabe H
Timmis K N
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23 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.43.1.391-396.1984
Published
1984-01-00
Pages
391-6
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC263439
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.43.1.391-396.1984 PMC Full Text View journal details
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