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PMID: 8164651 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Suppression of yeast RNA polymerase III mutations by FHL1, a gene coding for a fork head protein involved in rRNA processing.

Molecular and cellular biology ·Vol. 14 ·No. 5 ·1994-05-00 ·Pages 2905-13

Hermann-Le Denmat S, Werner M, Sentenac A, Thuriaux P

Abstract

The FHL1 gene was isolated by screening for high-copy-number suppressors of conditional RNA polymerase III mutations. This gene is unique on the yeast genome and was located close to RPC40 and PRE2 on the right arm of chromosome XVI. It codes for a 936-amino-acid protein containing a domain similar to the fork head DNA-binding domain, initially found in the developmental fork head protein of Drosophila melanogaster and in the HNF-3 family of hepatocyte mammalian transcription factors. Null mutations caused a severe reduction in growth rate and a lower rRNA content that resulted from defective rRNA processing. There was no detectable effect on mRNA splicing. Thus, the Fhl1p protein plays a key role in the control of rRNA processing, presumably by acting as a transcriptional regulator of genes specifically involved in that process. Moreover, mutants carrying the RNA polymerase III mutations were slightly defective in rRNA processing. This accounts for the isolation of FHL1 as a dosage-dependent suppressor and suggests that rRNA processing depends on a still-unidentified RNA polymerase III transcript.

Related Genes
FHL1 PRC40 PRE2
MeSH Terms
Amino Acid Sequence Base Sequence Chromosome Mapping Chromosomes, Fungal Cloning, Molecular Conserved Sequence Forkhead Transcription Factors Genes, Fungal Genome, Fungal Genotype Molecular Sequence Data Mutagenesis Mutation Nuclear Proteins/genetics Oligodeoxyribonucleotides RNA Polymerase III/biosynthesis,genetics RNA Processing, Post-Transcriptional RNA, Ribosomal/biosynthesis Restriction Mapping Saccharomyces cerevisiae/enzymology,genetics Sequence Homology, Amino Acid Suppression, Genetic Transcription Factors/genetics
Chemicals
Forkhead Transcription Factors Nuclear Proteins Oligodeoxyribonucleotides RNA, Ribosomal Transcription Factors RNA Polymerase III
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hermann-Le Denmat S
Département de Biologie Moléculaire et Cellulaire, Centre d'Etudes de Saclay, Gif-sur-Yvette, France.
Werner M
Sentenac A
Thuriaux P
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.14.5.2905-2913.1994
Published
1994-05-00
Pages
2905-13
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC358658
Subset
IM
Databases
GENBANK
J03177, L08252, L09647, L09648, L11148, M87634, S93559, X55955, X60787, X68662, Z28348
SWISSPROT
Z28348
External Links
PubMed source DOI: 10.1128/mcb.14.5.2905-2913.1994 PMC Full Text View journal details
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