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PMID: 8510644 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A general suppressor of RNA polymerase I, II and III mutations in Saccharomyces cerevisiae.

Molecular & general genetics : MGG ·Vol. 239 ·No. 1-2 ·1993-05-00 ·Pages 169-76

Stettler S, Chiannilkulchai N, Hermann-Le Denmat S, Lalo D, Lacroute F, Sentenac A, Thuriaux P

Abstract

A multicopy genomic library of Saccharomyces cerevisiae (strain FL100) was screened for its ability to suppress conditionally defective mutations altering the 31 kDa subunit (rpc31-236) or the 53 kDa subunit (rpc53-254/424) of RNA polymerase III. In addition to allele-specific suppressors, we identified seven suppressor clones that acted on both mutations and also suppressed several other conditional mutations defective in RNA polymerases I or II. All these clones harbored a complete copy of the SSD1 gene. The same pleiotropic suppression pattern was found with the dominant SSD1-v allele present in some laboratory strains of S. cerevisiae. SSD1-v was previously shown to suppress mutations defective in the SIT4 gene product (a predicted protein phosphatase subunit) or in the regulatory subunit of the cyclic AMP-dependent protein kinase. We propose that the SSD1 gene product modulates the activity (or the level) of the three nuclear RNA polymerases, possibly by altering their degree of phosphorylation.

Related Genes
SSD1
MeSH Terms
Alleles Fungal Proteins/genetics Genes, Fungal RNA Polymerase I/genetics RNA Polymerase II/genetics RNA Polymerase III/genetics Restriction Mapping Saccharomyces cerevisiae/enzymology,genetics Suppression, Genetic
Chemicals
Fungal Proteins RNA Polymerase II RNA Polymerase I RNA Polymerase III
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Stettler S
Département de Biologie Cellulaire et Moléculaire, C.E.A. Centre d'Etudes de Saclay, Gif sur Yvette, France.
Chiannilkulchai N
Hermann-Le Denmat S
Lalo D
Lacroute F
Sentenac A
Thuriaux P
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00281615
Published
1993-05-00
Pages
169-76
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00281615
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