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PMID: 8516295 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Interactions between three common subunits of yeast RNA polymerases I and III.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 90 ·No. 12 ·1993-06-15 ·Pages 5524-8

Lalo D, Carles C, Sentenac A, Thuriaux P

Abstract

The AC40 and AC19 subunits (encoded by RPC40 and RPC19) are shared by yeast RNA polymerases I and III and have a local sequence similarity to prokaryotic alpha subunits. Mutational analysis of the corresponding "alpha motif" indicated that its integrity is essential on AC40 subunit but is not essential on AC19 subunit. By applying the two-hybrid method, these two polypeptides were shown to associate in vivo. Extragenic suppression of rpc19 and rpc40 mutations confirmed that AC19 and AC40 subunits interact with each other in vivo and revealed an interaction with ABC10 beta subunit [encoded by RPB10; Woychick, N. A. & Young, R.A. (1990) J. Biol. Chem. 265, 17816-17819], one of the five polypeptides common to all three nuclear RNA polymerases. A correction of the RPB10 sequence showed that ABC10 beta subunit is a 70-amino acid polypeptide, as confirmed by peptide microsequencing. These results suggest that the assembly of RNA polymerase I and III requires the association of ABC10 beta subunit with an AC19/AC40 heterodimer.

Related Genes
PRB10 RPC19 RPC40
MeSH Terms
Amino Acid Sequence Animals Base Sequence Genes, Fungal Genetic Markers Genotype Humans Macromolecular Substances Molecular Sequence Data Mutagenesis, Site-Directed Plasmids RNA Polymerase I/genetics,metabolism RNA Polymerase III/genetics,metabolism Restriction Mapping Saccharomyces cerevisiae/enzymology,genetics Sequence Homology, Amino Acid
Chemicals
Genetic Markers Macromolecular Substances RNA Polymerase I RNA Polymerase III
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Lalo D
Département de Biologie Cellulaire et Moléculaire, Centre d'Etudes de Saclay, Gif sur Yvette, France.
Carles C
Sentenac A
Thuriaux P
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48 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.90.12.5524
Published
1993-06-15
Pages
5524-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC46753
Subset
IM
Databases
GENBANK
L11274, L11275
External Links
PubMed source DOI: 10.1073/pnas.90.12.5524 PMC Full Text View journal details
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