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PMID: 1406624 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

RPC53 encodes a subunit of Saccharomyces cerevisiae RNA polymerase C (III) whose inactivation leads to a predominantly G1 arrest.

Molecular and cellular biology ·Vol. 12 ·No. 10 ·1992-10-00 ·Pages 4314-26

Mann C, Micouin JY, Chiannilkulchai N, Treich I, Buhler JM, Sentenac A

Abstract

RPC53 is shown to be an essential gene encoding the C53 subunit specifically associated with yeast RNA polymerase C (III). Temperature-sensitive rpc53 mutants were generated and showed a rapid inhibition of tRNA synthesis after transfer to the restrictive temperature. Unexpectedly, the rpc53 mutants preferentially arrested their cell division in the G1 phase as large, round, unbudded cells. The RPC53 DNA sequence is predicted to code for a hydrophilic M(r)-46,916 protein enriched in charged amino acid residues. The carboxy-terminal 136 amino acids of C53 are significantly similar (25% identical amino acid residues) to the same region of the human BN51 protein. The BN51 cDNA was originally isolated by its ability to complement a temperature-sensitive hamster cell mutant that undergoes a G1 cell division arrest, as is true for the rpc53 mutants.

Related Genes
RPC53
MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular DNA, Fungal Flow Cytometry G1 Phase/genetics Genes, Fungal Kinetics Molecular Sequence Data Mutagenesis RNA Polymerase III/antagonists & inhibitors,genetics,metabolism RNA, Transfer/biosynthesis Restriction Mapping Saccharomyces cerevisiae/cytology,enzymology,genetics Sequence Homology Temperature Transcription, Genetic
Chemicals
DNA, Fungal RNA, Transfer RNA Polymerase III
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Mann C
Service de Biochimie et Génétique Moléculaire, Centre d'Etudes de Saclay, Gif-sur-Yvette, France.
Micouin J Y
Chiannilkulchai N
Treich I
Buhler J M
Sentenac A
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.12.10.4314-4326.1992
Published
1992-10-00
Pages
4314-26
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC360355
Subset
IM
Databases
GENBANK
X63501
External Links
PubMed source DOI: 10.1128/mcb.12.10.4314-4326.1992 PMC Full Text View journal details
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