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PMID: 3115591 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

A new class of yeast transcriptional activators.

Cell ·Vol. 51 ·No. 1 ·1987-10-09 ·Pages 113-9

Ma J, Ptashne M

Abstract

We describe yeast transcriptional activators encoded by E. coli genomic DNA fragments fused to the coding sequence of the DNA-binding portion of GAL4. All of the new activating sequences that we have analyzed, like those of GAL4 and GCN4, are acidic; most of these sequences show no obvious sequence homology when compared with the identified activating regions of GAL4 and GCN4 or among themselves. We also describe a fusion protein that contains no yeast protein sequence but activates transcription in yeast.

MeSH Terms
Amino Acid Sequence Binding Sites DNA/metabolism DNA, Bacterial/genetics DNA, Recombinant DNA-Binding Proteins/genetics Escherichia coli/genetics Fungal Proteins/genetics,metabolism Galactose/metabolism,pharmacology Molecular Sequence Data Plasmids Protein Kinases Recombinant Fusion Proteins/pharmacology Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Sequence Homology, Nucleic Acid Transcription Factors/genetics,metabolism Transcription, Genetic/drug effects beta-Galactosidase/genetics
Chemicals
DNA, Bacterial DNA, Recombinant DNA-Binding Proteins Fungal Proteins GAL4 protein, S cerevisiae Recombinant Fusion Proteins Saccharomyces cerevisiae Proteins Transcription Factors DNA Protein Kinases beta-Galactosidase Galactose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ma J
Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138.
Ptashne M
Article Info
Journal
Cell
Abbr.
Cell
ISSN
0092-8674
DOI
10.1016/0092-8674(87)90015-8
Published
1987-10-09
Pages
113-9
Language
English
Region
United States
NLM ID
0413066
Subset
IM
Grants
NIGMS NIH HHS · GM32308 · United States
Databases
GENBANK
M17537
Corrections
CommentIn
PMID: 15055587
CommentIn
PMID: 15055589
External Links
PubMed source DOI: 10.1016/0092-8674(87)90015-8 View journal details
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