Home LiteratureArticle Details
PMID: 1505518 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A novel gene, spp91-1, suppresses the splicing defect and the pre-mRNA nuclear export in the prp9-1 mutant.

The EMBO journal ·Vol. 11 ·No. 9 ·1992-09-00 ·Pages 3279-88

Chapon C, Legrain P

Abstract

Processing and export of nuclear pre-mRNA are believed to be competing processes in the nucleus. In order to identify factors which are involved in these processes, we isolated suppressors that relieve the growth defect of a prp9-1 temperature-sensitive mutant strain of Saccharomyces cerevisiae. The prp9-1 mutation was previously shown to abolish splicing and to target pre-mRNA to the cytoplasm. One of the suppressors, spp91-1, corrects the prp9-1 growth defect through partial restoration of splicing and by a complete reversion of the pre-mRNA escape phenotype. This suppressor is specific for two prp9 alleles and cannot substitute for PRP9 function. The mutant and wild-type alleles of SPP91 were cloned and sequenced. SPP91 encodes a novel protein essential for mitotic growth whose sequence contains motifs indicative of a nuclear localization. In vivo depletion of SPP91 in a prp9-1 genetic background is lethal and is associated with reduced amounts of spliced mRNA and accumulation of pre-mRNA. This observation strongly supports the hypothesis that SPP91 encodes a PRP factor. We suggest that spp91-1 increases pre-mRNA retention in the nucleus by improving the formation of the spliceosome and thereby allowing a larger proportion of the pre-mRNA molecules to be spliced.

Related Genes
spp91-1
MeSH Terms
Alleles Amino Acid Sequence Base Sequence Biological Transport Cell Nucleus/metabolism Cloning, Molecular DNA Mutational Analysis DNA, Fungal Genes, Fungal/genetics Models, Genetic Molecular Sequence Data Phenotype RNA Precursors/genetics,metabolism RNA Splicing RNA, Fungal/genetics,metabolism Restriction Mapping Saccharomyces cerevisiae/genetics,metabolism Suppression, Genetic
Chemicals
DNA, Fungal RNA Precursors RNA, Fungal
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Chapon C
Département de Biologie Moléculaire, Institut Pasteur, Paris, France.
Legrain P
References (63)
63 references, click to expand
  1. Cloning of the two essential yeast genes, PRP6 and PRP9, and their rapid mapping, disruption and partial sequencing using a linker insertion strategy.
    Mol Gen Genet. 1991 Feb;225(2):199-202 PMID: 1848649
  2. Expression of a beta-galactosidase gene containing the ribosomal protein 51 intron is sensitive to the rna2 mutation of yeast.
    Proc Natl Acad Sci U S A. 1983 Jul;80(14):4403-7 PMID: 6308621
  3. A cold-sensitive mRNA splicing mutant is a member of the RNA helicase gene family.
    Genes Dev. 1991 Apr;5(4):629-41 PMID: 2010088
  4. The molecular characterization of PRP6 and PRP9 yeast genes reveals a new cysteine/histidine motif common to several splicing factors.
    EMBO J. 1990 Sep;9(9):2775-81 PMID: 2118103
  5. Universally conserved and yeast-specific U1 snRNA sequences are important but not essential for U1 snRNP function.
    Genes Dev. 1990 Oct;4(10):1766-74 PMID: 2147421
  6. Targeted snRNP depletion reveals an additional role for mammalian U1 snRNP in spliceosome assembly.
    Cell. 1990 Oct 19;63(2):293-302 PMID: 2170025
  7. PRP5: a helicase-like protein required for mRNA splicing in yeast.
    Proc Natl Acad Sci U S A. 1990 Jun;87(11):4236-40 PMID: 2349233
  8. Identification of functional U1 snRNA-pre-mRNA complexes committed to spliceosome assembly and splicing.
    Cell. 1989 Oct 20;59(2):349-58 PMID: 2529976
  9. Some cis- and trans-acting mutants for splicing target pre-mRNA to the cytoplasm.
    Cell. 1989 May 19;57(4):573-83 PMID: 2655924
  10. Nuclear pre-mRNA splicing in yeast.
    Yeast. 1989 Nov-Dec;5(6):439-57 PMID: 2694677
  11. 'DNA Strider': a 'C' program for the fast analysis of DNA and protein sequences on the Apple Macintosh family of computers.
    Nucleic Acids Res. 1988 Mar 11;16(5):1829-36 PMID: 2832831
  12. Electrophoretic separation of complexes involved in the splicing of precursors to mRNAs.
    Cell. 1986 Sep 12;46(6):845-55 PMID: 2944598
  13. An early hierarchic role of U1 small nuclear ribonucleoprotein in spliceosome assembly.
    Science. 1988 Nov 18;242(4881):1028-35 PMID: 2973660
  14. Early commitment of yeast pre-mRNA to the spliceosome pathway.
    Mol Cell Biol. 1988 Sep;8(9):3755-60 PMID: 3065622
  15. Evidence for related functions of the RNA genes of Saccharomyces cerevisiae.
    Genetics. 1987 Dec;117(4):619-31 PMID: 3322934
  16. Evidence that a regulatory gene autoregulates splicing of its transcript.
    EMBO J. 1987 Dec 20;6(13):4105-11 PMID: 3443103
  17. mRNA splicing efficiency in yeast and the contribution of nonconserved sequences.
    Cell. 1985 May;41(1):119-26 PMID: 3888403
  18. DNA sequence required for efficient transcription termination in yeast.
    Cell. 1982 Mar;28(3):563-73 PMID: 6280875
  19. One-step gene disruption in yeast.
    Methods Enzymol. 1983;101:202-11 PMID: 6310324
  20. The effect of temperature-sensitive RNA mutants on the transcription products from cloned ribosomal protein genes of yeast.
    Cell. 1981 Jun;24(3):679-86 PMID: 7018694
  21. Replacement of chromosome segments with altered DNA sequences constructed in vitro.
    Proc Natl Acad Sci U S A. 1979 Oct;76(10):4951-5 PMID: 388424
  22. Use of polymerase chain reaction for rapid detection of gene insertions in whole yeast cells.
    Nucleic Acids Res. 1991 Sep 11;19(17):4775 PMID: 1653951
  23. Nuclear targeting sequences--a consensus?
    Trends Biochem Sci. 1991 Dec;16(12):478-81 PMID: 1664152
  24. A family of low and high copy replicative, integrative and single-stranded S. cerevisiae/E. coli shuttle vectors.
    Yeast. 1991 Aug-Sep;7(6):609-15 PMID: 1767589
  25. The yeast branchpoint sequence is not required for the formation of a stable U1 snRNA-pre-mRNA complex and is recognized in the absence of U2 snRNA.
    EMBO J. 1991 May;10(5):1209-16 PMID: 1827069
  26. An ATP-independent complex commits pre-mRNA to the mammalian spliceosome assembly pathway.
    Genes Dev. 1991 Dec;5(12B):2534-46 PMID: 1836445
  27. High efficiency transformation of intact yeast cells by electric field pulses.
    Biotechnology (N Y). 1990 Mar;8(3):223-7 PMID: 1366502
  28. Cloning and domain structure of the mammalian splicing factor U2AF.
    Nature. 1992 Feb 13;355(6361):609-14 PMID: 1538748
  29. Sequence of a 10.7 kb segment of yeast chromosome XI identifies the APN1 and the BAF1 loci and reveals one tRNA gene and several new open reading frames including homologs to RAD2 and kinases.
    Yeast. 1992 Feb;8(2):121-32 PMID: 1561835
  30. Arginine/serine-rich domains of the su(wa) and tra RNA processing regulators target proteins to a subnuclear compartment implicated in splicing.
    Cell. 1991 Oct 18;67(2):335-42 PMID: 1655279
  31. RNA processing is a limiting step for murine tumor necrosis factor beta expression in response to interleukin-2.
    Mol Cell Biol. 1990 Nov;10(11):5865-75 PMID: 1700275
  32. Nuclear RNA processing.
    Curr Opin Cell Biol. 1991 Jun;3(3):493-501 PMID: 1716447
  33. Nuclear mRNA export.
    Curr Opin Cell Biol. 1991 Dec;3(6):1004-12 PMID: 1814360
  34. Three protein factors (SF1, SF3 and U2AF) function in pre-splicing complex formation in addition to snRNPs.
    EMBO J. 1991 Jun;10(6):1503-9 PMID: 1827409
  35. Suppressors of a U4 snRNA mutation define a novel U6 snRNP protein with RNA-binding motifs.
    Genes Dev. 1991 May;5(5):773-85 PMID: 1827420
  36. Biochemical mechanisms of constitutive and regulated pre-mRNA splicing.
    Annu Rev Cell Biol. 1991;7:559-99 PMID: 1839712
  37. Messenger RNA splicing in yeast: clues to why the spliceosome is a ribonucleoprotein.
    Science. 1991 Jul 12;253(5016):157-63 PMID: 1853200
  38. A suppressor of a yeast splicing mutation (prp8-1) encodes a putative ATP-dependent RNA helicase.
    Nature. 1991 Feb 21;349(6311):715-7 PMID: 1996139
  39. Cloning genes by complementation in yeast.
    Methods Enzymol. 1991;194:195-230 PMID: 2005788
  40. Pre-mRNA splicing in yeast.
    Trends Genet. 1991 Mar;7(3):79-85 PMID: 2031287
  41. Splicing stories and poly(A) tales: an update on RNA processing and transport.
    Curr Opin Cell Biol. 1990 Jun;2(3):528-38 PMID: 2143075
  42. The yeast PRP6 gene encodes a U4/U6 small nuclear ribonucleoprotein particle (snRNP) protein, and the PRP9 gene encodes a protein required for U2 snRNP binding.
    Mol Cell Biol. 1990 Dec;10(12):6417-25 PMID: 2147224
  43. The PRP4 (RNA4) protein of Saccharomyces cerevisiae is associated with the 5' portion of the U4 small nuclear RNA.
    Mol Cell Biol. 1990 Mar;10(3):1217-25 PMID: 2154681
  44. U1 small nuclear RNA plays a direct role in the formation of a rev-regulated human immunodeficiency virus env mRNA that remains unspliced.
    Proc Natl Acad Sci U S A. 1990 Oct;87(19):7598-602 PMID: 2217190
  45. PRP4 (RNA4) from Saccharomyces cerevisiae: its gene product is associated with the U4/U6 small nuclear ribonucleoprotein particle.
    Mol Cell Biol. 1989 Sep;9(9):3698-709 PMID: 2528686
  46. PRP4: a protein of the yeast U4/U6 small nuclear ribonucleoprotein particle.
    Mol Cell Biol. 1989 Sep;9(9):3710-9 PMID: 2528687
  47. Identification, purification, and biochemical characterization of U2 small nuclear ribonucleoprotein auxiliary factor.
    Proc Natl Acad Sci U S A. 1989 Dec;86(23):9243-7 PMID: 2531895
  48. Isolation and characterization of pre-mRNA splicing mutants of Saccharomyces cerevisiae.
    Genes Dev. 1989 Aug;3(8):1206-16 PMID: 2676722
  49. Regulation by HIV Rev depends upon recognition of splice sites.
    Cell. 1989 Dec 1;59(5):789-95 PMID: 2686839
  50. Developmental expression of a regulatory gene is programmed at the level of splicing.
    EMBO J. 1987 Dec 20;6(13):4095-104 PMID: 2832151
  51. A trans-acting suppressor restores splicing of a yeast intron with a branch point mutation.
    Genes Dev. 1987 Jul;1(5):445-55 PMID: 2890553
  52. Spliceosome assembly in yeast.
    Genes Dev. 1987 Nov;1(9):1014-27 PMID: 2962902
  53. A factor, U2AF, is required for U2 snRNP binding and splicing complex assembly.
    Cell. 1988 Jan 29;52(2):207-19 PMID: 2963698
  54. RNA11 protein is associated with the yeast spliceosome and is localized in the periphery of the cell nucleus.
    Mol Cell Biol. 1988 Jun;8(6):2379-93 PMID: 3043176
  55. Sequences responsible for transcription termination on a gene segment in Saccharomyces cerevisiae.
    Mol Cell Biol. 1984 Aug;4(8):1515-20 PMID: 6436686
  56. A GAL10-CYC1 hybrid yeast promoter identifies the GAL4 regulatory region as an upstream site.
    Proc Natl Acad Sci U S A. 1982 Dec;79(23):7410-4 PMID: 6760197
  57. Presplicing complex formation requires two proteins and U2 snRNP.
    Genes Dev. 1988 Sep;2(9):1155-67 PMID: 3192077
  58. A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformation of Escherichia coli.
    Gene. 1987;57(2-3):267-72 PMID: 3319781
  59. 5-Fluoroorotic acid as a selective agent in yeast molecular genetics.
    Methods Enzymol. 1987;154:164-75 PMID: 3323810
  60. Electrophoresis of ribonucleoproteins reveals an ordered assembly pathway of yeast splicing complexes.
    Nature. 1986 Nov 27-Dec 3;324(6095):341-5 PMID: 3537805
  61. Identification of ten genes that control ribosome formation in yeast.
    Mol Gen Genet. 1970;109(1):42-56 PMID: 5488085
  62. Two genes for ribosomal protein 51 of Saccharomyces cerevisiae complement and contribute to the ribosomes.
    Mol Cell Biol. 1984 Sep;4(9):1871-9 PMID: 6092944
  63. The complete sequence of the 8.2 kb segment left of MAT on chromosome III reveals five ORFs, including a gene for a yeast ribokinase.
    Yeast. 1990 Nov-Dec;6(6):521-34 PMID: 1964349
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1992-09-00
Pages
3279-88
Language
English
Region
England
NLM ID
8208664
PMCID
PMC556862
Subset
IM
Databases
GENBANK
X66121, X66358, X66359, X66360, X66361, X66362, X66363, X66364, X66365, X67564
External Links
PubMed source PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2