Use of polymerase chain reaction for rapid detection of gene insertions in whole yeast cells.

Nucleic acids research ·Vol. 19 ·No. 17 ·1991-09-11 ·Pages 4775

Sathe GM, O'Brien S, McLaughlin MM, Watson F, Livi GP

Abstract

暂无摘要

MeSH Terms

Base Sequence Blotting, Southern Chromosome Deletion DNA Restriction Enzymes/metabolism DNA, Single-Stranded/genetics Molecular Sequence Data Mutation/genetics Polymerase Chain Reaction/methods Saccharomyces cerevisiae/genetics Transformation, Genetic

Chemicals

DNA, Single-Stranded DNA Restriction Enzymes

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Sathe G M

Department of Molecular Genetics, Smith Kline Beecham Pharmaceuticals, King of Prussia, PA 19406.

O'Brien S

McLaughlin M M

Watson F

Livi G P

References (5)

5 references, click to expand

Rapamycin sensitivity in Saccharomyces cerevisiae is mediated by a peptidyl-prolyl cis-trans isomerase related to human FK506-binding protein.
Mol Cell Biol. 1991 Mar;11(3):1718-23 PMID: 1996117
Direct clone characterization from plaques and colonies by the polymerase chain reaction.
Nucleic Acids Res. 1989 May 25;17(10):4000 PMID: 2734114
One-step gene disruption in yeast.
Methods Enzymol. 1983;101:202-11 PMID: 6310324
Structure and function of the yeast URA3 gene: expression in Escherichia coli.
Gene. 1984 Jul-Aug;29(1-2):113-24 PMID: 6092217
Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction.
Methods Enzymol. 1987;155:335-50 PMID: 3431465

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/19.17.4775

Published

1991-09-11

Pages

4775

Language

English

Region

England

NLM ID

0411011

PMCID

PMC328729

Subset

External Links

PubMed source DOI: 10.1093/nar/19.17.4775 PMC Full Text View journal details

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Use of polymerase chain reaction for rapid detection of gene insertions in whole yeast cells.

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