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PMID: 8026462 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Characterization of two protein kinases from Schizosaccharomyces pombe involved in the regulation of DNA repair.

The EMBO journal ·Vol. 13 ·No. 12 ·1994-06-15 ·Pages 2777-88

Dhillon N, Hoekstra MF

Abstract

We have identified two novel genes designated hhp1+ and hhp2+ in the fission yeast Schizosaccharomyces pombe. The hhp1+ and hhp2+ genes encode two closely related protein kinases that share significant sequence identities with Hrr25p from Saccharomyces cerevisiae. Characterization of strains harboring single and double mutations in the hhp+ genes reveals DNA repair defects in these cells. Schizosaccharomyces pombe strains lacking either or both Hhp activities reveal differences in their ability to withstand DNA lesions caused by either methyl methanesulfonate (MMS) or gamma-rays which correlate with their ability to repair DNA strand breaks caused by these agents. We suggest that Hhp1 and Hhp2 are involved in the regulation of distinct and overlapping DNA repair pathways in S. pombe.

Related Genes
hhp1<up>+</up> hhp2<up>+</up>
MeSH Terms
Amino Acid Sequence Base Sequence Casein Kinase I Casein Kinases DNA Repair DNA, Fungal/radiation effects Escherichia coli/genetics Fungal Proteins/genetics Gamma Rays Genes, Fungal/genetics Genetic Complementation Test Isoenzymes/genetics Molecular Sequence Data Mutagenesis, Insertional Protein Kinases/genetics,metabolism Recombinant Proteins/metabolism Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Schizosaccharomyces/enzymology,genetics Schizosaccharomyces pombe Proteins Sequence Homology, Amino Acid
Chemicals
DNA, Fungal Fungal Proteins Isoenzymes Recombinant Proteins Saccharomyces cerevisiae Proteins Schizosaccharomyces pombe Proteins Protein Kinases hhp1 protein, S pombe hhp2 protein, S pombe Casein Kinase I Casein Kinases HRR25 protein, S cerevisiae
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Dhillon N
Molecular Biology and Virology Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037.
Hoekstra M F
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1994-06-15
Pages
2777-88
Language
English
Region
England
NLM ID
8208664
PMCID
PMC395157
Subset
IM
Databases
GENBANK
X78871, X78872
External Links
PubMed source PMC Full Text View journal details
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