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PMID: 1627830 Published · ppublish English Journal Article

Two genes in Saccharomyces cerevisiae encode a membrane-bound form of casein kinase-1.

Molecular biology of the cell ·Vol. 3 ·No. 3 ·1992-03-00 ·Pages 275-86

Wang PC, Vancura A, Mitcheson TG, Kuret J

Abstract

Two cDNAs encoding casein kinase-1 have been isolated from a yeast cDNA library and termed CKI1 and CKI2. Each clone encodes a protein of approximately 62,000 Da containing a highly conserved protein kinase domain surrounded by variable amino- and carboxy-terminal domains. The proteins also contain two conserved carboxy-terminal cysteine residues that comprise a consensus sequence for prenylation. Consistent with this posttranslational modification, cell fractionation experiments demonstrate that intact CKI1 is found exclusively in yeast cell membranes. Gene disruption experiments reveal that, although neither of the two CKI genes is essential by itself, at least one CKI gene is required for yeast cell viability. Spores deficient in both CKI1 and CKI2 fail to grow and, therefore, either fail to germinate or arrest as small cells before bud emergence. These results suggest that casein kinase-1, which is distributed widely in nature, plays a pivotal role in eukaryotic cell regulation.

MeSH Terms
Amino Acid Sequence Base Sequence Casein Kinases Cell Membrane/enzymology Chromosome Mapping Cloning, Molecular DNA, Fungal/chemistry,genetics,isolation & purification Genes, Fungal/genetics Molecular Sequence Data Phenotype Protein Kinases/chemistry,genetics,isolation & purification Saccharomyces cerevisiae/enzymology,genetics
Chemicals
DNA, Fungal Protein Kinases Casein Kinases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Wang P C
Cold Spring Harbor Laboratory, New York 11724.
Vancura A
Mitcheson T G
Kuret J
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.3.3.275
Published
1992-03-00
Pages
275-86
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC275529
Subset
IM
Databases
GENBANK
X60326, X60327
External Links
PubMed source DOI: 10.1091/mbc.3.3.275 PMC Full Text View journal details
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