Home LiteratureArticle Details
PMID: 1946367 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Purification of casein kinase I and isolation of cDNAs encoding multiple casein kinase I-like enzymes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 21 ·1991-11-01 ·Pages 9548-52

Rowles J, Slaughter C, Moomaw C, Hsu J, Cobb MH

Abstract

We have purified casein kinase I (CKI) over 6000-fold from bovine thymus and have sequenced seven tryptic peptides that account for nearly 25% of the primary sequence of the enzyme. By using PCR, partial cDNAs encoding CKI and a related enzyme (CKI-delta) were isolated. A product that may correspond to an alternatively spliced form of CKI was also detected. The CKI PCR product was used to probe a bovine brain cDNA library from which cDNAs corresponding to CKI (CKI-alpha) and two homologous enzymes (CKI-beta and CKI-gamma) were identified. The finding that there are at least four CKI-like enzymes suggests that CKI activity in tissues or cell extracts may be composed of multiple related but distinct protein kinases. This group of enzymes is not similar to any other known protein kinases and may, therefore, represent an additional branch of the protein kinase family.

Related Genes
CKI
MeSH Terms
Amino Acid Sequence Animals Base Sequence Blotting, Northern Casein Kinases Caseins/metabolism Cattle DNA/genetics Gene Expression Molecular Sequence Data Oligonucleotides/chemistry Protein Biosynthesis Protein Kinases/genetics,isolation & purification RNA, Messenger/genetics Sequence Alignment Thymus Gland/chemistry Transcription, Genetic
Chemicals
Caseins Oligonucleotides RNA, Messenger DNA Protein Kinases Casein Kinases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Rowles J
University of Texas Southwestern Medical Center, Department of Pharmacology, Dallas 75235-9041.
Slaughter C
Moomaw C
Hsu J
Cobb M H
References (30)
30 references, click to expand
  1. Phosphoprotein kinases from rat liver cytosol.
    Biochim Biophys Acta. 1972 Nov 10;289(1):237-41 PMID: 5086948
  2. HRR25, a putative protein kinase from budding yeast: association with repair of damaged DNA.
    Science. 1991 Aug 30;253(5023):1031-4 PMID: 1887218
  3. Purification of rat liver nuclear protein kinase NI.
    J Biol Chem. 1978 Jul 10;253(13):4638-41 PMID: 207704
  4. Cyclic nucleotide-independent protein kinases from rabbit reticulocytes. Purification of casein kinases.
    J Biol Chem. 1979 Feb 10;254(3):762-8 PMID: 216682
  5. Studies on the structural requirements of a microsomal cAMP-independent protein kinase.
    FEBS Lett. 1979 Oct 1;106(1):76-80 PMID: 227729
  6. Purification and characterization of a membrane-bound protein kinase from human erythrocytes.
    J Biol Chem. 1980 Mar 25;255(6):2563-8 PMID: 7358688
  7. Purification and properties of calf thymus casein kinases I and II.
    J Biol Chem. 1981 Apr 10;256(7):3319-25 PMID: 6937462
  8. Phosphorylation of rat ascites tumor non-histone chromatin proteins. Differential phosphorylation by two cyclic nucleotide-independent protein kinases and comparison to in vivo phosphorylation.
    J Biol Chem. 1981 Apr 10;256(7):3326-31 PMID: 6937463
  9. Cyclic adenosine 3':5'-monophosphate-dependent and -independent protein kinase in acute myeloblastic leukemia.
    Cancer Res. 1981 Jun;41(6):2182-8 PMID: 6263462
  10. Description of a protein kinase derived from insulin-treated 3T3-L1 cells that catalyzes the phosphorylation of ribosomal protein S6 and casein.
    J Biol Chem. 1983 Oct 25;258(20):12472-81 PMID: 6313661
  11. Glycogen synthase kinases. Classification of a rabbit liver casein and glycogen synthase kinase (casein kinase-1) as a distinct enzyme.
    J Biol Chem. 1984 Mar 25;259(6):3420-8 PMID: 6323424
  12. Purification and characterization of a nuclear protein kinase from rat liver and a hepatoma that is capable of activating poly(A) polymerase.
    J Biol Chem. 1984 Dec 10;259(23):14481-5 PMID: 6094560
  13. Phosphorylation of ankyrin decreases its affinity for spectrin tetramer.
    J Biol Chem. 1985 Dec 5;260(28):14958-64 PMID: 2933395
  14. An insulin-stimulated ribosomal protein S6 kinase in 3T3-L1 cells.
    J Biol Chem. 1986 Oct 5;261(28):12994-9 PMID: 3531196
  15. Molecular characterization of cell cycle gene CDC7 from Saccharomyces cerevisiae.
    Mol Cell Biol. 1986 May;6(5):1590-8 PMID: 3537706
  16. Formation of protein kinase recognition sites by covalent modification of the substrate. Molecular mechanism for the synergistic action of casein kinase II and glycogen synthase kinase 3.
    J Biol Chem. 1987 Oct 15;262(29):14042-8 PMID: 2820993
  17. Changes in protein kinase activities in lamb adrenals at late gestation and early postnatal stages.
    Mol Cell Endocrinol. 1987 Oct;53(3):195-202 PMID: 2822512
  18. An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs.
    Nucleic Acids Res. 1987 Oct 26;15(20):8125-48 PMID: 3313277
  19. Internal amino acid sequence analysis of proteins separated by one- or two-dimensional gel electrophoresis after in situ protease digestion on nitrocellulose.
    Proc Natl Acad Sci U S A. 1987 Oct;84(20):6970-4 PMID: 3313383
  20. Isolation and sequencing of cDNA clones encoding alpha and beta subunits of Drosophila melanogaster casein kinase II.
    Mol Cell Biol. 1987 Oct;7(10):3409-17 PMID: 3119988
  21. Developmental changes in rat hepatic casein kinases 1 and 2.
    Eur J Biochem. 1987 Dec 30;170(1-2):493-8 PMID: 3121327
  22. The protein kinase family: conserved features and deduced phylogeny of the catalytic domains.
    Science. 1988 Jul 1;241(4861):42-52 PMID: 3291115
  23. Synergistic phosphorylation of rabbit muscle glycogen synthase by cyclic AMP-dependent protein kinase and casein kinase I. Implications for hormonal regulation of glycogen synthase.
    J Biol Chem. 1989 Jun 5;264(16):9126-8 PMID: 2498326
  24. Erythroid membrane-bound protein kinase binds to a membrane component and is regulated by phosphatidylinositol 4,5-bisphosphate.
    J Biol Chem. 1990 May 5;265(13):7369-76 PMID: 2158999
  25. Molecular cloning and expression of glycogen synthase kinase-3/factor A.
    EMBO J. 1990 Aug;9(8):2431-8 PMID: 2164470
  26. Linearization of baculovirus DNA enhances the recovery of recombinant virus expression vectors.
    Nucleic Acids Res. 1990 Oct 11;18(19):5667-72 PMID: 2216760
  27. Casein kinase I is regulated by phosphatidylinositol 4,5-bisphosphate in native membranes.
    J Biol Chem. 1991 Feb 5;266(4):2508-12 PMID: 1846628
  28. Casein kinase I and II--multipotential serine protein kinases: structure, function, and regulation.
    Adv Second Messenger Phosphoprotein Res. 1991;23:123-64 PMID: 1997039
  29. Principal neurofilament-associated protein kinase in squid axoplasm is related to casein kinase I.
    J Biol Chem. 1991 Mar 15;266(8):4987-94 PMID: 2002043
  30. Purification and properties of rat liver nuclear protein kinases.
    Can J Biochem. 1972 Dec;50(12):1249-59 PMID: 4345911
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.21.9548
Published
1991-11-01
Pages
9548-52
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC52755
Subset
IM
Grants
NIDDK NIH HHS · DK34128 · United States
NIGMS NIH HHS · GM07062 · United States
NIGMS NIH HHS · GM44140 · United States
Databases
GENBANK
M76542, M76543, M76544, M76545, S60073, S60076, S60079, S60081, S64934, S68289
External Links
PubMed source DOI: 10.1073/pnas.88.21.9548 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2