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PMID: 7889932 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Crystal structure of casein kinase-1, a phosphate-directed protein kinase.

The EMBO journal ·Vol. 14 ·No. 5 ·1995-03-01 ·Pages 1015-23

Xu RM, Carmel G, Sweet RM, Kuret J, Cheng X

Abstract

The structure of a truncated variant of casein kinase-1 from Schizosaccharomyces pombe, has been determined in complex with MgATP at 2.0 A resolution. The model resembles the 'closed', ATP-bound conformations of the cyclin-dependent kinase 2 and the cAMP-dependent protein kinase, with clear differences in the structure of surface loops that impart unique features to casein kinase-1. The structure is of unphosphorylated, active conformation of casein kinase-1 and the peptide-binding site is fully accessible to substrate.

MeSH Terms
Adenosine Triphosphate/metabolism Amino Acid Sequence Binding Sites Casein Kinases Crystallography, X-Ray Models, Molecular Molecular Sequence Data Phosphorylation Protein Conformation Protein Kinases/chemistry,genetics,metabolism Schizosaccharomyces/enzymology Sequence Alignment Sequence Deletion Substrate Specificity
Chemicals
Adenosine Triphosphate Protein Kinases Casein Kinases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Xu R M
W.M. Keck Structural Biology Laboratory, Cold Spring Harbor, NY 11724.
Carmel G
Sweet R M
Kuret J
Cheng X
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1995-03-01
Pages
1015-23
Language
English
Region
England
NLM ID
8208664
PMCID
PMC398173
Subset
IM
Grants
NIGMS NIH HHS · GM 44806 · United States
NIGMS NIH HHS · GM 48216 · United States
External Links
PubMed source PMC Full Text View journal details
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