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PMID: 1409656 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Cell cycle-dependent localization of casein kinase I to mitotic spindles.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 20 ·1992-10-15 ·Pages 9454-8

Brockman JL, Gross SD, Sussman MR, Anderson RA

Abstract

Casein kinase I (CKI) is a class of protein kinases ubiquitous to all eukaryotic cells. Recently, cDNA clones encoding several bovine CKI isoforms have been sequenced that show high sequence identity to the HRR25 gene product of the budding yeast Saccharomyces cerevisiae; HRR25 is required for normal cellular growth, nuclear segregation, DNA repair, and meiosis. We have raised polyclonal antibodies to a human erythroid 34-kDa CKI and have sequenced a portion of this kinase. The amino acid sequence identifies the CKI as the alpha-CKI isoform, which is 62% identical to the HRR25 protein kinase. By use of immunofluorescence, the alpha-CKI has been localized to vesicular cytosolic structures and to the centrosome in interphase cells. As cells progress into mitosis, centrospheric staining increases and, in mitosis, alpha-CKI associates with kinetochore fibers. This localization suggests that alpha-CKI, like HRR25, plays a role in the segregation of chromosomes during mitosis and may be cell cycle-regulated both in humans and in yeast.

MeSH Terms
Amino Acid Sequence Casein Kinases Cell Cycle Erythrocytes/chemistry Fluorescent Antibody Technique Humans Molecular Sequence Data Protein Kinases/immunology,metabolism Sequence Alignment Spindle Apparatus/enzymology
Chemicals
Protein Kinases Casein Kinases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Brockman J L
Department of Pharmacology, University of Wisconsin Medical School, Madison 53706.
Gross S D
Sussman M R
Anderson R A
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.20.9454
Published
1992-10-15
Pages
9454-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC50150
Subset
IM
Grants
NIGMS NIH HHS · R01 GM38906-06 · United States
External Links
PubMed source DOI: 10.1073/pnas.89.20.9454 PMC Full Text View journal details
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