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PMID: 2686978 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

p34cdc2 is located in both nucleus and cytoplasm; part is centrosomally associated at G2/M and enters vesicles at anaphase.

The EMBO journal ·Vol. 8 ·No. 13 ·1989-12-20 ·Pages 3985-95

Bailly E, Dorée M, Nurse P, Bornens M

Abstract

The cdc2+ gene product p34cdc2 is located immunocytochemically in both the nucleus and cytoplasm of human cells. It is uniformly distributed throughout the cytoplasm and is irregularly distributed in the nucleus. Part of p34cdc2 is associated with the centrosome and centrosomal staining increases late in the cell cycle and at the onset of mitosis. This distribution is corroborated by cell fractionation which also indicates that slower migrating forms of p34cdc2 are found in isolated centrosomes and in Triton-insoluble fractions. We propose that one role of the p34cdc2 protein kinase is to modify the centrosome bringing about formation of the mitotic spindle. At anaphase p34cdc2 becomes associated with vesicles in the middle of the cell between the reforming nuclei. A similar location is found for p13suc1 and we suggest that the vesicular localization plays a role in p34cdc2 kinase inactivation at the end of mitosis.

MeSH Terms
Amino Acid Sequence Anaphase Antibodies Blotting, Western CDC2 Protein Kinase Cell Cycle Cell Fractionation/methods Cell Nucleus/enzymology Cytoplasm/enzymology Fluorescent Antibody Technique HeLa Cells/cytology,enzymology Humans Interphase Molecular Sequence Data Phosphoproteins/analysis,immunology,metabolism Protein Kinases/analysis
Chemicals
Antibodies Phosphoproteins Protein Kinases CDC2 Protein Kinase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Bailly E
Centre de Génétique Moléculaire, CNRS, Gif/Yvette, France.
Dorée M
Nurse P
Bornens M
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1989-12-20
Pages
3985-95
Language
English
Region
England
NLM ID
8208664
PMCID
PMC401573
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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