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PMID: 3283736 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Purification of maturation-promoting factor, an intracellular regulator of early mitotic events.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 9 ·1988-05-00 ·Pages 3009-13

Lohka MJ, Hayes MK, Maller JL

Abstract

Maturation-promoting factor causes germinal vesicle breakdown when injected into Xenopus oocytes and can induce metaphase in a cell-free system. The cell-free assay was used to monitor maturation-promoting factor during its purification from unfertilized Xenopus eggs. Ammonium sulfate precipitation and six chromatographic procedures resulted in a preparation purified greater than 3000-fold that could induce germinal vesicle breakdown within 2 hr when injected into cycloheximide-treated oocytes. Proteins of 45 kDa and 32 kDa were correlated with fractions of highest activity in both assays. These fractions contained a protein kinase activity able to phosphorylate the endogenous 45-kDa protein, as well as histone H1, phosphatase inhibitor 1, and casein. The highly purified preparations described here should help to identify the mechanism of action of maturation-promoting factor and to elucidate the role of protein kinases in the induction of metaphase.

MeSH Terms
Animals Chromatography Cycloheximide/pharmacology Growth Substances/isolation & purification Maturation-Promoting Factor Mitosis Oocytes/drug effects Xenopus laevis
Chemicals
Growth Substances Cycloheximide Maturation-Promoting Factor
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lohka M J
Department of Pharmacology, University of Colorado School of Medicine, Denver 80262.
Hayes M K
Maller J L
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34 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.9.3009
Published
1988-05-00
Pages
3009-13
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC280132
Subset
IM
Grants
NIGMS NIH HHS · GM 26743 · United States
External Links
PubMed source DOI: 10.1073/pnas.85.9.3009 PMC Full Text View journal details
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