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PMID: 1557394 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A protein kinase associated with paired helical filaments in Alzheimer disease.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 7 ·1992-04-01 ·Pages 2878-82

Vincent IJ, Davies P

Abstract

We have identified a protein kinase in immunoaffinity-purified preparations of paired helical filaments from brain tissue of individuals with Alzheimer disease. The kinase phosphorylates the filament proteins in vitro in a manner independent of second messenger regulation or of modulation by heparin and polyamines. Physiological concentrations of hemin, an oxidized heme porphyrin, inhibit the kinase and abolish Alz-50 immunoreactivity of the proteins. Since paired helical filaments are composed of hyperphosphorylated proteins, association of a protein kinase with the filaments provides a mechanism for abnormal processing of the proteins in disease.

MeSH Terms
Adenosine Triphosphate/metabolism Alzheimer Disease/enzymology Antibodies, Monoclonal Cell Compartmentation Hemin/pharmacology Kinetics Microscopy, Electron Neurofibrillary Tangles/enzymology Phosphoproteins/metabolism Phosphorylation Protein Kinase Inhibitors Protein Kinases/metabolism Substrate Specificity tau Proteins/metabolism
Chemicals
Antibodies, Monoclonal Phosphoproteins Protein Kinase Inhibitors tau Proteins Hemin Adenosine Triphosphate Protein Kinases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Vincent I J
Department of Pathology, Albert Einstein College of Medicine, Bronx, NY 10461.
Davies P
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.7.2878
Published
1992-04-01
Pages
2878-82
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC48766
Subset
IM
Grants
NIA NIH HHS · AG06803 · United States
NIMH NIH HHS · MH38623 · United States
External Links
PubMed source DOI: 10.1073/pnas.89.7.2878 PMC Full Text View journal details
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