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PMID: 7738195 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Mycobacterium tuberculosis enhances human immunodeficiency virus-1 replication by transcriptional activation at the long terminal repeat.

The Journal of clinical investigation ·Vol. 95 ·No. 5 ·1995-05-00 ·Pages 2324-31

Zhang Y, Nakata K, Weiden M, Rom WN

Abstract

Tuberculosis has emerged as an epidemic fueled by the large number of individuals infected with the human immunodeficiency virus, especially those who are injecting drug users. We found a striking increase from 4- to 208-fold in p24 levels in bronchoalveolar lavage fluid from involved sites of Mycobacterium tuberculosis infection vs uninvolved sites in three HIV+ patients. We used an in vitro cell culture model to determine if tuberculosis could activate replication of HIV-1. Mononuclear phagocyte cell lines U937 and THP-1 infected with HIV-1JR-CSF, in vitro and stimulated with live M. tuberculosis H37Ra, had a threefold increase in p24 in culture supernatants. Using the HIV-1 long terminal repeat with a chloramphenicol acetyltransferase (CAT) reporter construct, live M. tuberculosis increased transcription 20-fold in THP-1 cells, and cell wall components stimulated CAT expression to a lesser extent. The nuclear factor-kappa B enhancer element was responsible for the majority of the increased CAT activity although two upstream nuclear factor-IL6 sites may also contribute to enhanced transcription. Antibodies to TNF-alpha and IL-1 inhibited the increase in CAT activity of the HIV-1 long terminal repeat by M. tuberculosis from 21-fold to 8-fold. Stimulation of HIV-1 replication by M. tuberculosis may exacerbate dysfunction of the host immune response in dually infected individuals.

MeSH Terms
AIDS-Related Opportunistic Infections/virology Adult Antibodies/pharmacology Base Sequence CCAAT-Enhancer-Binding Proteins Cell Line Cell Nucleus/metabolism Chloramphenicol O-Acetyltransferase/analysis,biosynthesis DNA-Binding Proteins/metabolism HIV Core Protein p24/analysis,biosynthesis HIV Long Terminal Repeat HIV Seropositivity/virology HIV-1/genetics,physiology Humans Interleukin-1/physiology Molecular Sequence Data Mutagenesis, Site-Directed Mycobacterium tuberculosis/physiology NF-kappa B/metabolism Nuclear Proteins/metabolism Oligodeoxyribonucleotides Transcription Factors/metabolism Transcription, Genetic Transcriptional Activation Transfection Tuberculosis/virology Tumor Necrosis Factor-alpha/physiology Virus Replication
Chemicals
Antibodies CCAAT-Enhancer-Binding Proteins DNA-Binding Proteins HIV Core Protein p24 Interleukin-1 NF-kappa B Nuclear Proteins Oligodeoxyribonucleotides Transcription Factors Tumor Necrosis Factor-alpha Chloramphenicol O-Acetyltransferase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Zhang Y
Department of Medicine, New York University Medical Center, New York 10016, USA.
Nakata K
Weiden M
Rom W N
References (56)
56 references, click to expand
  1. Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.
    Mol Cell Biol. 1982 Sep;2(9):1044-51 PMID: 6960240
  2. The emergence of drug-resistant tuberculosis in New York City.
    N Engl J Med. 1993 Feb 25;328(8):521-6 PMID: 8381207
  3. Direct stimulation of monocyte release of interleukin 1 by mycobacterial protein antigens.
    J Immunol. 1986 Jan;136(1):193-6 PMID: 3484390
  4. Estimation of volume of epithelial lining fluid recovered by lavage using urea as marker of dilution.
    J Appl Physiol (1985). 1986 Feb;60(2):532-8 PMID: 3512509
  5. Release of interleukin-1 by peripheral blood mononuclear cells in patients with tuberculosis and active inflammation.
    Infect Immun. 1986 Apr;52(1):341-3 PMID: 3485575
  6. Inducibility of kappa immunoglobulin enhancer-binding protein Nf-kappa B by a posttranslational mechanism.
    Cell. 1986 Dec 26;47(6):921-8 PMID: 3096580
  7. Enhanced susceptibility of blood monocytes from patients with pulmonary tuberculosis to productive infection with human immunodeficiency virus type 1.
    J Exp Med. 1993 May 1;177(5):1511-6 PMID: 8478621
  8. Mechanisms of stimulation of interleukin-1 beta and tumor necrosis factor-alpha by Mycobacterium tuberculosis components.
    J Clin Invest. 1993 May;91(5):2076-83 PMID: 7683696
  9. Regulation of the interleukin-1 beta (IL-1 beta) gene by mycobacterial components and lipopolysaccharide is mediated by two nuclear factor-IL6 motifs.
    Mol Cell Biol. 1993 Jun;13(6):3831-7 PMID: 7684503
  10. Early molecular replication of human immunodeficiency virus type 1 in cultured-blood-derived T helper dendritic cells.
    J Clin Invest. 1993 Jun;91(6):2721-6 PMID: 8514880
  11. Thalidomide inhibits the replication of human immunodeficiency virus type 1.
    Proc Natl Acad Sci U S A. 1993 Jul 1;90(13):5974-8 PMID: 8327469
  12. The NF-kappa B and Sp1 motifs of the human immunodeficiency virus type 1 long terminal repeat function as novel thyroid hormone response elements.
    Mol Cell Biol. 1993 Aug;13(8):5057-69 PMID: 8393143
  13. Cytokine gene activation and modified responsiveness to interleukin-2 in the blood of tuberculosis patients.
    J Infect Dis. 1993 Oct;168(4):1056-9 PMID: 8376820
  14. Distinct mechanisms for regulation of the interleukin-8 gene involve synergism and cooperativity between C/EBP and NF-kappa B.
    Mol Cell Biol. 1993 Nov;13(11):7191-8 PMID: 8413306
  15. Transcription factors NF-IL6 and NF-kappa B synergistically activate transcription of the inflammatory cytokines, interleukin 6 and interleukin 8.
    Proc Natl Acad Sci U S A. 1993 Nov 1;90(21):10193-7 PMID: 8234276
  16. Relationship of the manifestations of tuberculosis to CD4 cell counts in patients with human immunodeficiency virus infection.
    Am Rev Respir Dis. 1993 Nov;148(5):1292-7 PMID: 7902049
  17. Structure and antigenicity of lipoarabinomannan from Mycobacterium bovis BCG.
    J Gen Microbiol. 1993 Nov;139(11):2649-58 PMID: 8277248
  18. HIV-1 in human alveolar macrophages from infected patients is latent in vivo but replicates after in vitro stimulation.
    Am J Respir Cell Mol Biol. 1994 Jan;10(1):72-8 PMID: 8292383
  19. Activation of the interleukin 6 gene by Mycobacterium tuberculosis or lipopolysaccharide is mediated by nuclear factors NF-IL6 and NF-kappa B.
    Proc Natl Acad Sci U S A. 1994 Mar 15;91(6):2225-9 PMID: 8134378
  20. CD8+ lymphocytes can control HIV infection in vitro by suppressing virus replication.
    Science. 1986 Dec 19;234(4783):1563-6 PMID: 2431484
  21. An inducible transcription factor activates expression of human immunodeficiency virus in T cells.
    Nature. 1987 Apr 16-22;326(6114):711-3 PMID: 3031512
  22. Human T-cell lymphotropic virus-III (HTLV-III) seropositivity and related disease among 71 consecutive patients in whom tuberculosis was diagnosed. A prospective study.
    Am Rev Respir Dis. 1987 Apr;135(4):875-9 PMID: 3645999
  23. Tuberculosis in patients with the acquired immunodeficiency syndrome. Clinical features, response to therapy, and survival.
    Am Rev Respir Dis. 1987 Sep;136(3):570-4 PMID: 3631730
  24. Human immunodeficiency virus long terminal repeat responds to T-cell activation signals.
    Proc Natl Acad Sci U S A. 1987 Oct;84(19):6845-9 PMID: 3498942
  25. Interaction between Mycobacterium avium and human immunodeficiency virus type 1 (HIV-1) in bronchoalveolar macrophages of normal and HIV-1-infected subjects.
    Am J Respir Cell Mol Biol. 1994 Oct;11(4):487-95 PMID: 7917317
  26. C/EBP beta regulation of the tumor necrosis factor alpha gene.
    J Clin Invest. 1994 Oct;94(4):1449-55 PMID: 7929820
  27. Accelerated course of human immunodeficiency virus infection after tuberculosis.
    Am J Respir Crit Care Med. 1995 Jan;151(1):129-35 PMID: 7812542
  28. Characterization of cellular proteins recognizing the HIV enhancer using a microscale DNA-affinity precipitation assay.
    Nature. 1987 Nov 26-Dec 2;330(6146):391-5 PMID: 2825023
  29. Anti-termination of transcription within the long terminal repeat of HIV-1 by tat gene product.
    Nature. 1987 Dec 3-9;330(6147):489-93 PMID: 2825027
  30. Activation of the HIV-1 LTR by T cell mitogens and the trans-activator protein of HTLV-I.
    Science. 1987 Dec 11;238(4833):1575-8 PMID: 2825351
  31. Cytokines alter production of HIV-1 from primary mononuclear phagocytes.
    Science. 1988 Sep 23;241(4873):1673-5 PMID: 3047875
  32. Monokine regulation of human immunodeficiency virus-1 expression in a chronically infected human T cell clone.
    J Immunol. 1989 Jan 15;142(2):431-8 PMID: 2463307
  33. A prospective study of the risk of tuberculosis among intravenous drug users with human immunodeficiency virus infection.
    N Engl J Med. 1989 Mar 2;320(9):545-50 PMID: 2915665
  34. The inducing role of tumor necrosis factor in the development of bactericidal granulomas during BCG infection.
    Cell. 1989 Mar 10;56(5):731-40 PMID: 2647299
  35. Tumor necrosis factor alpha and interleukin 1 stimulate the human immunodeficiency virus enhancer by activation of the nuclear factor kappa B.
    Proc Natl Acad Sci U S A. 1989 Apr;86(7):2336-40 PMID: 2494664
  36. Lipoarabinomannan from Mycobacterium tuberculosis induces the production of tumour necrosis factor from human and murine macrophages.
    Clin Exp Immunol. 1989 May;76(2):240-5 PMID: 2503277
  37. Tumor necrosis factor alpha activates human immunodeficiency virus type 1 through induction of nuclear factor binding to the NF-kappa B sites in the long terminal repeat.
    Proc Natl Acad Sci U S A. 1989 Aug;86(15):5974-8 PMID: 2762307
  38. Induction of expression of human immunodeficiency virus in a chronically infected promonocytic cell line by ultraviolet irradiation.
    AIDS Res Hum Retroviruses. 1989 Aug;5(4):375-84 PMID: 2475151
  39. Tumor necrosis factor alpha functions in an autocrine manner in the induction of human immunodeficiency virus expression.
    Proc Natl Acad Sci U S A. 1990 Jan;87(2):782-5 PMID: 2300561
  40. Induction of interleukin 1 and tumor necrosis factor by mycobacterial proteins: the monocyte western blot.
    Proc Natl Acad Sci U S A. 1990 May;87(9):3348-52 PMID: 2110362
  41. Human immunodeficiency virus infection in tuberculosis patients.
    J Infect Dis. 1990 Jul;162(1):8-12 PMID: 1972384
  42. Interleukin 6 induces human immunodeficiency virus expression in infected monocytic cells alone and in synergy with tumor necrosis factor alpha by transcriptional and post-transcriptional mechanisms.
    J Exp Med. 1990 Jul 1;172(1):151-8 PMID: 2193094
  43. Production of tumor necrosis factor alpha by monocytes from patients with pulmonary tuberculosis.
    Infect Immun. 1990 Oct;58(10):3286-92 PMID: 2205576
  44. The molecular biology of human immunodeficiency virus type 1 infection.
    N Engl J Med. 1991 Jan 31;324(5):308-17 PMID: 1986289
  45. The influence of human immunodeficiency virus infection on tuberculosis in Kampala, Uganda.
    Am Rev Respir Dis. 1991 Jan;143(1):185-7 PMID: 1986677
  46. Tuberculosis in patients with human immunodeficiency virus infection.
    N Engl J Med. 1991 Jun 6;324(23):1644-50 PMID: 2030721
  47. Immunopathogenesis of HIV infection.
    FASEB J. 1991 Jul;5(10):2382-90 PMID: 1676689
  48. Ionizing radiation induces expression and binding activity of the nuclear factor kappa B.
    J Clin Invest. 1991 Aug;88(2):691-5 PMID: 1864978
  49. Increase in tumor necrosis factor alpha- and interleukin-6-secreting cells in peripheral blood mononuclear cells from subjects infected with Mycobacterium tuberculosis.
    Infect Immun. 1991 Sep;59(9):3021-5 PMID: 1879927
  50. Disseminated tuberculosis in the acquired immunodeficiency syndrome era.
    Am Rev Respir Dis. 1991 Nov;144(5):1164-70 PMID: 1952449
  51. Extrapulmonary tuberculosis in patients with human immunodeficiency virus infection.
    Medicine (Baltimore). 1991 Nov;70(6):384-97 PMID: 1956280
  52. An outbreak of tuberculosis with accelerated progression among persons infected with the human immunodeficiency virus. An analysis using restriction-fragment-length polymorphisms.
    N Engl J Med. 1992 Jan 23;326(4):231-5 PMID: 1345800
  53. Tuberculous meningitis in patients infected with the human immunodeficiency virus.
    N Engl J Med. 1992 Mar 5;326(10):668-72 PMID: 1346547
  54. Cytokine production induced by Mycobacterium tuberculosis lipoarabinomannan. Relationship to chemical structure.
    J Immunol. 1992 Jul 15;149(2):541-7 PMID: 1624801
  55. Tuberculosis: commentary on a reemergent killer.
    Science. 1992 Aug 21;257(5073):1055-64 PMID: 1509256
  56. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
    Nucleic Acids Res. 1983 Mar 11;11(5):1475-89 PMID: 6828386
Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI117924
Published
1995-05-00
Pages
2324-31
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC295846
Subset
IM
Grants
NIAID NIH HHS · AI 35233 · United States
NHLBI NIH HHS · HL-51494 · United States
NCRR NIH HHS · MO1 RR00096 · United States
External Links
PubMed source DOI: 10.1172/JCI117924 PMC Full Text View journal details
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