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PMID: 2193094 Published · ppublish English Journal Article

Interleukin 6 induces human immunodeficiency virus expression in infected monocytic cells alone and in synergy with tumor necrosis factor alpha by transcriptional and post-transcriptional mechanisms.

The Journal of experimental medicine ·Vol. 172 ·No. 1 ·1990-07-01 ·Pages 151-8

Poli G, Bressler P, Kinter A, Duh E, Timmer WC, Rabson A, Justement JS, Stanley S, Fauci AS

Abstract

The immunoregulatory cytokine interleukin 6 (IL-6) directly upregulates production of human immunodeficiency virus (HIV) in acutely as well as in chronically infected cells of monocytic lineage. In addition, IL-6 synergizes with tumor necrosis factor alpha (TNF-alpha) in the induction of latent HIV expression. Unlike TNF-alpha, upregulation of viral expression induced by IL-6 alone does not occur at the transcriptional level and it is not associated with accumulation of HIV RNA. However, when IL-6 and TNF-alpha synergistically stimulate HIV production, accumulation of HIV RNA and increased transcription are observed, indicating that IL-6 affects HIV expression at multiple (transcriptional and post-transcriptional) levels.

MeSH Terms
Blotting, Northern Blotting, Western Cell Line Colony-Stimulating Factors/pharmacology Drug Synergism Gene Expression Regulation, Viral/drug effects Granulocyte-Macrophage Colony-Stimulating Factor Growth Substances/pharmacology HIV-1/drug effects,growth & development Humans Interleukin-6/pharmacology Monocytes/microbiology RNA, Viral/biosynthesis Recombinant Proteins Retroviridae Proteins/biosynthesis Transcription, Genetic/drug effects Tumor Necrosis Factor-alpha/pharmacology Virus Activation/drug effects
Chemicals
Colony-Stimulating Factors Growth Substances Interleukin-6 RNA, Viral Recombinant Proteins Retroviridae Proteins Tumor Necrosis Factor-alpha Granulocyte-Macrophage Colony-Stimulating Factor
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Poli G
Laboratories of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892.
Bressler P
Kinter A
Duh E
Timmer W C
Rabson A
Justement J S
Stanley S
Fauci A S
References (34)
34 references, click to expand
  1. T cell-replacing factor- (TRF) induced IgG secretion in a human B blastoid cell line and demonstration of acceptors for TRF.
    J Immunol. 1981 Aug;127(2):412-6 PMID: 6972960
  2. Tumor necrosis factor alpha functions in an autocrine manner in the induction of human immunodeficiency virus expression.
    Proc Natl Acad Sci U S A. 1990 Jan;87(2):782-5 PMID: 2300561
  3. Susceptibility of normal human lymphocytes to infection with HTLV-III/LAV.
    J Immunol. 1986 Jun 1;136(11):4049-53 PMID: 2422271
  4. A macrophage-derived factor required by plasmacytomas for survival and proliferation in vitro.
    Science. 1986 Aug 1;233(4763):566-9 PMID: 3726549
  5. Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone.
    J Virol. 1986 Aug;59(2):284-91 PMID: 3016298
  6. Structural analysis of the sequence coding for an inducible 26-kDa protein in human fibroblasts.
    Eur J Biochem. 1986 Sep 15;159(3):625-32 PMID: 3758081
  7. Structure and expression of cDNA and genes for human interferon-beta-2, a distinct species inducible by growth-stimulatory cytokines.
    EMBO J. 1986 Oct;5(10):2529-37 PMID: 3023045
  8. Identification of the human 26-kD protein, interferon beta 2 (IFN-beta 2), as a B cell hybridoma/plasmacytoma growth factor induced by interleukin 1 and tumor necrosis factor.
    J Exp Med. 1987 Mar 1;165(3):914-9 PMID: 3493322
  9. Interferon beta 2/B-cell stimulatory factor type 2 shares identity with monocyte-derived hepatocyte-stimulating factor and regulates the major acute phase protein response in liver cells.
    Proc Natl Acad Sci U S A. 1987 Oct;84(20):7251-5 PMID: 2444978
  10. Cytokine-induced expression of HIV-1 in a chronically infected promonocyte cell line.
    Science. 1987 Nov 6;238(4828):800-2 PMID: 3313729
  11. In vitro mutagenesis identifies a region within the envelope gene of the human immunodeficiency virus that is critical for infectivity.
    J Virol. 1988 Jan;62(1):139-47 PMID: 3257102
  12. Transcriptional regulation of the interferon-beta 2/B cell differentiation factor BSF-2/hepatocyte-stimulating factor gene in human fibroblasts by other cytokines.
    J Immunol. 1988 Feb 1;140(3):974-7 PMID: 3276786
  13. Characterization of a promonocyte clone chronically infected with HIV and inducible by 13-phorbol-12-myristate acetate.
    J Immunol. 1988 Feb 15;140(4):1117-22 PMID: 2449497
  14. Effect of neuropeptides on production of inflammatory cytokines by human monocytes.
    Science. 1988 Sep 2;241(4870):1218-21 PMID: 2457950
  15. Cytokines alter production of HIV-1 from primary mononuclear phagocytes.
    Science. 1988 Sep 23;241(4873):1673-5 PMID: 3047875
  16. Cachectin/tumor necrosis factor exerts endocrine, paracrine, and autocrine control of inflammatory responses.
    J Cell Biol. 1988 Oct;107(4):1269-77 PMID: 3049617
  17. Monokine regulation of human immunodeficiency virus-1 expression in a chronically infected human T cell clone.
    J Immunol. 1989 Jan 15;142(2):431-8 PMID: 2463307
  18. Induction of IL-6 (B cell stimulatory factor-2/IFN-beta 2) production by HIV.
    J Immunol. 1989 Jan 15;142(2):531-6 PMID: 2783441
  19. IL-1 stimulates IL-6 production in endothelial cells.
    J Immunol. 1989 Jan 15;142(2):549-53 PMID: 2783442
  20. Tumor necrosis factor alpha and interleukin 1 stimulate the human immunodeficiency virus enhancer by activation of the nuclear factor kappa B.
    Proc Natl Acad Sci U S A. 1989 Apr;86(7):2336-40 PMID: 2494664
  21. Tumor necrosis factor alpha induces expression of human immunodeficiency virus in a chronically infected T-cell clone.
    Proc Natl Acad Sci U S A. 1989 Apr;86(7):2365-8 PMID: 2784570
  22. Augmentation of human immunodeficiency virus type 1 gene expression by tumor necrosis factor alpha.
    AIDS Res Hum Retroviruses. 1989 Apr;5(2):131-8 PMID: 2713164
  23. Interferon-alpha but not AZT suppresses HIV expression in chronically infected cell lines.
    Science. 1989 May 5;244(4904):575-7 PMID: 2470148
  24. Human immunodeficiency virus type 1 (HIV-1) infection of the central nervous system: an evaluation of cytokines in cerebrospinal fluid.
    J Neuroimmunol. 1989 Jul;23(2):109-16 PMID: 2656753
  25. Viral antigen stimulation of the production of human monokines capable of regulating HIV1 expression.
    J Immunol. 1989 Jul 15;143(2):470-5 PMID: 2544645
  26. The reservoir for HIV-1 in human peripheral blood is a T cell that maintains expression of CD4.
    Science. 1989 Jul 21;245(4915):305-8 PMID: 2665081
  27. The biology of interleukin-6.
    Blood. 1989 Jul;74(1):1-10 PMID: 2473791
  28. Tumor necrosis factor alpha activates human immunodeficiency virus type 1 through induction of nuclear factor binding to the NF-kappa B sites in the long terminal repeat.
    Proc Natl Acad Sci U S A. 1989 Aug;86(15):5974-8 PMID: 2762307
  29. Multiple actions of interleukin 6 within a cytokine network.
    Immunol Today. 1988 May;9(5):137-9 PMID: 3076767
  30. Interleukin-1 and tumor necrosis factor alpha can be induced from mononuclear phagocytes by human immunodeficiency virus type 1 binding to the CD4 receptor.
    J Virol. 1989 Oct;63(10):4404-8 PMID: 2789293
  31. HIV-1, HTLV-1 and normal T-cell growth: transcriptional strategies and surprises.
    Immunol Today. 1989 Aug;10(8):272-8 PMID: 2679647
  32. Interleukin 6 induces secretion of IgG1 by coordinated transcriptional activation and differential mRNA accumulation.
    Proc Natl Acad Sci U S A. 1989 Oct;86(20):8024-8 PMID: 2813375
  33. Infection with HIV is associated with elevated IL-6 levels and production.
    J Immunol. 1990 Jan 15;144(2):480-4 PMID: 2295799
  34. Enhanced transcription of c-myc in bursal lymphoma cells requires continuous protein synthesis.
    Science. 1985 Dec 6;230(4730):1126-32 PMID: 2999973
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.172.1.151
Published
1990-07-01
Pages
151-8
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2188185
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.172.1.151 PMC Full Text View journal details
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