Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone.

Journal of virology ·Vol. 59 ·No. 2 ·1986-08-00 ·Pages 284-91

Adachi A, Gendelman HE, Koenig S, Folks T, Willey R, Rabson A, Martin MA

Abstract

We constructed an infectious molecular clone of acquired immunodeficiency syndrome-associated retrovirus. Upon transfection, this clone directed the production of infectious virus particles in a wide variety of cells in addition to human T4 cells. The progeny, infectious virions, were synthesized in mouse, mink, monkey, and several human non-T cell lines, indicating the absence of any intracellular obstacle to viral RNA or protein production or assembly. During the course of these studies, a human colon carcinoma cell line, exquisitely sensitive to DNA transfection, was identified.

MeSH Terms

Animals Cell Differentiation Cell Line Cloning, Molecular DNA, Viral/genetics Deltaretrovirus/genetics,growth & development Gene Expression Regulation Humans Lymphocytes/microbiology Receptors, Virus/physiology Species Specificity Transfection Virus Replication

Chemicals

DNA, Viral Receptors, Virus

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Adachi A

Gendelman H E

Koenig S

Folks T

Willey R

Rabson A

Martin M A

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38 references, click to expand

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Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.59.2.284-291.1986

Published

1986-08-00

Pages

284-91

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC253077

Subset

Databases

GENBANK

M19921

External Links

PubMed source DOI: 10.1128/JVI.59.2.284-291.1986 PMC Full Text View journal details

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Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone.

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