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PMID: 1864978 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Ionizing radiation induces expression and binding activity of the nuclear factor kappa B.

The Journal of clinical investigation ·Vol. 88 ·No. 2 ·1991-08-00 ·Pages 691-5

Brach MA, Hass R, Sherman ML, Gunji H, Weichselbaum R, Kufe D

Abstract

Recent studies have demonstrated that treatment of mammalian cells with ionizing radiation is associated with activation of gene expression. Although the signal transduction pathways stimulated by ionizing radiation remain unclear, our previous findings indicate that radiation induces specific genes at the transcriptional level. The present work has examined the effects of ionizing radiation on the transcription factor NF-kappa B. The results demonstrate that ionizing radiation activates DNA binding of nuclear factor (NF)kappa B. This effect was detectable at 2 grays (Gy) and reached a maximum at 5-20 Gy. At a dose of 20 Gy, the increase in NF-kappa B binding activity was maximal at 2-4 h and then declined to pretreatment levels. The results also demonstrate that ionizing radiation transiently increases NF-kappa B mRNA levels. However, the finding that induction of NF-kappa B binding to DNA occurs in the presence of cycloheximide indicates that ionizing radiation activates preexisting NF-kappa B protein. NF-kappa B exists as a cytoplasmic protein before activation. Thus, our results suggest that ionizing radiation induces transduction pathways which include cytoplasmic signaling events.

MeSH Terms
Base Sequence Blotting, Northern Cesium Radioisotopes Cycloheximide/pharmacology DNA/metabolism,radiation effects DNA Damage Gene Expression Regulation/radiation effects Humans Molecular Sequence Data NF-kappa B/metabolism,radiation effects Protein Kinase C/physiology Radiation, Ionizing Tumor Cells, Cultured
Chemicals
Cesium Radioisotopes NF-kappa B DNA Cycloheximide Protein Kinase C
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Brach M A
Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115.
Hass R
Sherman M L
Gunji H
Weichselbaum R
Kufe D
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI115354
Published
1991-08-00
Pages
691-5
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC295415
Subset
IM
Grants
NCI NIH HHS · CA29431 · United States
NCI NIH HHS · CA44068 · United States
External Links
PubMed source DOI: 10.1172/JCI115354 PMC Full Text View journal details
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