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PMID: 7682653 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A novel, erythroid cell-specific murine transcription factor that binds to the CACCC element and is related to the Krüppel family of nuclear proteins.

Molecular and cellular biology ·Vol. 13 ·No. 5 ·1993-05-00 ·Pages 2776-86

Miller IJ, Bieker JJ

Abstract

We describe a novel erythroid cell-specific cDNA (EKLF [erythroid Krüppel-like factor]) isolated by enriching for genes expressed in a mouse erythroleukemia cell line but not expressed in a mouse monocyte-macrophage cell line. The complete cDNA sequence is predicted to encode a protein of approximately 38,000 Da that contains a proline-rich amino domain and three TFIIIA-like zinc fingers within the carboxy domain. Additional sequence analyses reveal that the EKLF zinc fingers are most homologous to the Krüppel family of transcription factors and also allow us to predict potential DNA-binding target sites for the EKLF protein. On the basis of this prediction, we show that EKLF is able to bind the sequence CCA CAC CCT, an essential element of the beta-globin promoter. Its tissue distribution establishes that the EKLF transcript is expressed only in bone marrow and spleen, the two hematopoietic organs of the mouse, and analysis of murine cell lines indicates that EKLF expression is limited to erythroid and mast cell lines. Cotransfection assays establish that EKLF transcriptionally activates a target promoter that contains its DNA-binding site. The tissue expression pattern of EKLF, in conjunction with its function as a transcriptional activator, strongly suggests that the EKLF protein may be intimately involved in establishment and/or maintenance of the erythroid cell phenotype.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Blotting, Northern Cell Line Chromosome Mapping Cricetinae DNA-Binding Proteins/genetics,isolation & purification,metabolism Hybrid Cells Kruppel-Like Transcription Factors Leukemia, Erythroblastic, Acute Macrophages Mice Molecular Sequence Data Nuclear Proteins/genetics,metabolism Oligodeoxyribonucleotides Oligonucleotides, Antisense Polymerase Chain Reaction RNA/genetics,isolation & purification Repressor Proteins Sequence Homology, Amino Acid Substrate Specificity Transcription Factors/genetics,isolation & purification,metabolism Tumor Cells, Cultured Zinc Fingers/genetics,physiology
Chemicals
DNA-Binding Proteins Kruppel-Like Transcription Factors Nuclear Proteins Oligodeoxyribonucleotides Oligonucleotides, Antisense Repressor Proteins Transcription Factors erythroid Kruppel-like factor RNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Miller I J
Department of Biochemistry, Mount Sinai School of Medicine, New York, New York 10029.
Bieker J J
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.13.5.2776-2786.1993
Published
1993-05-00
Pages
2776-86
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC359658
Subset
IM
Grants
NIGMS NIH HHS · GM07280 · United States
NICHD NIH HHS · HD23250 · United States
Databases
GENBANK
M97200
External Links
PubMed source DOI: 10.1128/mcb.13.5.2776-2786.1993 PMC Full Text View journal details
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