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PMID: 2922281 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The purification of an erythroid protein which binds to enhancer and promoter elements of haemoglobin genes.

Nucleic acids research ·Vol. 17 ·No. 4 ·1989-02-25 ·Pages 1299-314

Perkins ND, Nicolas RH, Plumb MA, Goodwin GH

Abstract

An erythroid nuclear protein (EF1), originally detected as a protein binding within the nuclease hypersensitive site upstream of the chicken beta H-globin gene, has been purified. This protein of 37,000-39,000 molecular weight binds to three sites within the hypersensitive region: one between the CCAAT and TATA boxes, the second (further upstream) next to a NF1 binding site, and the third adjacent to a regulatory element found in a number of beta-globin genes. The EF1 protein also binds to an erythroid-specific promoter element of the mouse alpha-globin gene and to two sites within the chicken beta A-globin enhancer. These six EF1-binding sites are related by the consensus sequence A/TGATAA/GG/C. A minor protein of molecular weight 72,000 which co-purifies with EF1 also binds to the same sequences.

MeSH Terms
Animals Base Sequence Binding Sites Chickens Chromatography, Affinity Deoxyribonuclease I Enhancer Elements, Genetic Erythrocytes/metabolism Genes Globins/genetics Molecular Sequence Data Nuclear Proteins/blood Promoter Regions, Genetic Restriction Mapping
Chemicals
Nuclear Proteins Globins Deoxyribonuclease I
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Perkins N D
Institute of Cancer Research, Chester Beatty Laboratories, London, UK.
Nicolas R H
Plumb M A
Goodwin G H
References (28)
28 references, click to expand
  1. High resolution two-dimensional electrophoresis of proteins.
    J Biol Chem. 1975 May 25;250(10):4007-21 PMID: 236308
  2. TGGCA protein is present in erythroid nuclei and binds within the nuclease-hypersensitive sites 5' of the chicken beta H- and beta A-globin genes.
    Eur J Biochem. 1988 Nov 15;177(3):505-11 PMID: 3197715
  3. Temperature-sensitive changes in the structure of globin chromatin in lines of red cell precursors transformed by ts-AEV.
    Cell. 1982 Apr;28(4):931-40 PMID: 7094019
  4. Hemoglobin switching in chickens. Is the switch initiated post-transcriptionally?
    J Biol Chem. 1982 Sep 25;257(18):11008-14 PMID: 6179944
  5. An altered DNA conformation detected by S1 nuclease occurs at specific regions in active chick globin chromatin.
    Cell. 1982 Jun;29(2):609-22 PMID: 6288265
  6. Expression and partial DNA sequence of the chicken beta H-globin gene.
    J Biol Chem. 1983 Jan 25;258(2):802-9 PMID: 6296082
  7. Isolation of microgram quantities of proteins from polyacrylamide gels for amino acid sequence analysis.
    Methods Enzymol. 1983;91:227-36 PMID: 6855576
  8. Interaction of specific nuclear factors with the nuclease-hypersensitive region of the chicken adult beta-globin gene: nature of the binding domain.
    Cell. 1985 May;41(1):21-30 PMID: 2986843
  9. Regulated gene expression in transfected primary chicken erythrocytes.
    Proc Natl Acad Sci U S A. 1986 Jun;83(12):4312-6 PMID: 3459175
  10. Affinity purification of sequence-specific DNA binding proteins.
    Proc Natl Acad Sci U S A. 1986 Aug;83(16):5889-93 PMID: 3461465
  11. A 3' enhancer is required for temporal and tissue-specific transcriptional activation of the chicken adult beta-globin gene.
    Nature. 1986 Oct 23-29;323(6090):731-4 PMID: 3022151
  12. Characterisation of chicken erythroid nuclear proteins which bind to the nuclease hypersensitive regions upstream of the beta A- and beta H-globin genes.
    Nucleic Acids Res. 1986 Oct 10;14(19):7675-93 PMID: 3774543
  13. Sequences closely related to an immunoglobulin gene promoter/enhancer element occur also upstream of other eukaryotic and of prokaryotic genes.
    Nucleic Acids Res. 1986 Nov 25;14(22):8819-27 PMID: 3537963
  14. Protein-binding sites within the 5' DNase I-hypersensitive region of the chicken alpha D-globin gene.
    Mol Cell Biol. 1987 Jun;7(6):2059-69 PMID: 3600658
  15. Analysis of the tissue-specific enhancer at the 3' end of the chicken adult beta-globin gene.
    Proc Natl Acad Sci U S A. 1987 Jul;84(14):4786-90 PMID: 3474625
  16. Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes.
    J Biol Chem. 1987 Jul 25;262(21):10035-8 PMID: 3611052
  17. DNA affinity labeling of adenovirus type 2 upstream promoter sequence-binding factors identifies two distinct proteins.
    Mol Cell Biol. 1988 Jan;8(1):105-13 PMID: 3336354
  18. Detection of two tissue-specific DNA-binding proteins with affinity for sites in the mouse beta-globin intervening sequence 2.
    Mol Cell Biol. 1988 Jan;8(1):381-92 PMID: 3422099
  19. Chicken liver TGGCA protein purified by preparative mobility shift electrophoresis (PMSE) shows a 36.8 to 29.8 kd microheterogeneity.
    Nucleic Acids Res. 1987 Dec 10;15(23):9707-26 PMID: 3122180
  20. A transcriptional enhancer located between adult beta-globin and embryonic epsilon-globin genes in chicken and duck.
    Gene. 1987;58(2-3):167-75 PMID: 3428615
  21. A promoter of the rat insulin-like growth factor II gene consists of minimal control elements.
    J Mol Biol. 1988 Jan 5;199(1):61-81 PMID: 3351924
  22. Bidirectional control of the chicken beta- and epsilon-globin genes by a shared enhancer.
    Proc Natl Acad Sci U S A. 1988 Apr;85(8):2548-52 PMID: 3357880
  23. Preparative elution of proteins blotted to Immobilon membranes.
    Anal Biochem. 1988 Jan;168(1):48-53 PMID: 3364716
  24. The human beta-globin gene contains multiple regulatory regions: identification of one promoter and two downstream enhancers.
    EMBO J. 1988 Feb;7(2):377-84 PMID: 2835225
  25. Identification of three sequence-specific DNA-binding proteins which interact with the Rous sarcoma virus enhancer and upstream promoter elements.
    J Virol. 1988 Jun;62(6):2186-90 PMID: 2835519
  26. An erythrocyte-specific DNA-binding factor recognizes a regulatory sequence common to all chicken globin genes.
    Proc Natl Acad Sci U S A. 1988 Aug;85(16):5976-80 PMID: 3413070
  27. Mutational analysis of the chicken beta-globin enhancer reveals two positive-acting domains.
    Proc Natl Acad Sci U S A. 1988 Sep;85(17):6267-71 PMID: 3166139
  28. Hormone-dependent terminal differentiation in vitro of chicken erythroleukemia cells transformed by ts mutants of avian erythroblastosis virus.
    Cell. 1982 Apr;28(4):907-19 PMID: 6954007
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/17.4.1299
Published
1989-02-25
Pages
1299-314
Language
English
Region
England
NLM ID
0411011
PMCID
PMC331804
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/17.4.1299 PMC Full Text View journal details
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