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PMID: 3122180 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Chicken liver TGGCA protein purified by preparative mobility shift electrophoresis (PMSE) shows a 36.8 to 29.8 kd microheterogeneity.

Nucleic acids research ·Vol. 15 ·No. 23 ·1987-12-10 ·Pages 9707-26

Rupp RA, Sippel AE

Abstract

The TGGCA protein, the chicken homologue of HeLa cell NF-I, was purified to homogeneity from liver tissue by a procedure which includes preparative mobility shift electrophoresis (PMSE) as the final step. PMSE was here adjusted for the isolation of the TGGCA protein, but can be used as a general method to characterize the protein moiety of specific DNA-binding proteins. The TGGCA protein is a family of 6 protein species, which show minor differences in molecular weight from 36.8kd to 29.8kd. This microheterogeneity differs from the size distribution reported for HeLa cell NF-I polypeptides. All species of the TGGCA protein bind identically to a synthetic DNA-binding site and appear to be highly related in primary structure. We discuss the possible functional importance of this microheterogeneity.

MeSH Terms
Acrylamides Animals Binding, Competitive Boric Acids CCAAT-Enhancer-Binding Proteins Chickens DNA-Binding Proteins/isolation & purification,metabolism Edetic Acid Electrophoresis, Polyacrylamide Gel/methods Liver/analysis Molecular Weight NFI Transcription Factors Nuclear Proteins Peptide Fragments/isolation & purification Sodium Dodecyl Sulfate Transcription Factors Tromethamine Y-Box-Binding Protein 1
Chemicals
Acrylamides Boric Acids CCAAT-Enhancer-Binding Proteins DNA-Binding Proteins NFI Transcription Factors Nuclear Proteins Peptide Fragments Transcription Factors Y-Box-Binding Protein 1 YBX1 protein, human Tromethamine Sodium Dodecyl Sulfate Edetic Acid boric acid
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Rupp R A
Zentrum für Molekulare Biologie, Universität Heidelberg, FRG.
Sippel A E
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.23.9707
Published
1987-12-10
Pages
9707-26
Language
English
Region
England
NLM ID
0411011
PMCID
PMC306526
Subset
IM
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1093/nar/15.23.9707 PMC Full Text View journal details
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