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PMID: 3038519 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Interaction of the TGGCA-binding protein with upstream sequences is required for efficient transcription of mouse mammary tumor virus.

The EMBO journal ·Vol. 6 ·No. 5 ·1987-05-00 ·Pages 1355-60

Miksicek R, Borgmeyer U, Nowock J

Abstract

A high-affinity binding site for the TGGCA-binding protein, also known as nuclear factor I, has previously been shown to reside within the mouse mammary tumor virus (MMTV) long terminal repeat. We have introduced mutations into this binding site to test the importance of this ubiquitous nuclear protein in MMTV transcription. Mutations which abolish the binding of the TGGCA protein in vitro are shown to impair strongly glucocorticoid-induced transcription from this promoter in vivo. These data demonstrate that the TGGCA-binding protein is a multifunctional DNA-binding protein, capable of serving a transcriptional role in the case of MMTV, in addition to its known involvement in the replication of adenovirus.

MeSH Terms
Animals Base Sequence CCAAT-Enhancer-Binding Proteins Cell Line Cell Nucleus/metabolism DNA-Binding Proteins/physiology Genes, Viral Humans Liver/metabolism Mammary Tumor Virus, Mouse/genetics Mice NFI Transcription Factors Nuclear Proteins Protein Binding Proto-Oncogenes Transcription Factors Transcription, Genetic Y-Box-Binding Protein 1
Chemicals
CCAAT-Enhancer-Binding Proteins DNA-Binding Proteins NFI Transcription Factors Nuclear Proteins Transcription Factors Y-Box-Binding Protein 1 YBX1 protein, human
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Miksicek R
Borgmeyer U
Nowock J
References (33)
33 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1987-05-00
Pages
1355-60
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553940
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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