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PMID: 7637000 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Herpes simplex virus immediate-early protein ICP22 is required for viral modification of host RNA polymerase II and establishment of the normal viral transcription program.

Journal of virology ·Vol. 69 ·No. 9 ·1995-09-00 ·Pages 5550-9

Rice SA, Long MC, Lam V, Schaffer PA, Spencer CA

Abstract

Infection of cells with herpes simplex virus type 1 (HSV-1) results in a rapid alteration of phosphorylation on the large subunit of cellular RNA polymerase II (RNAP II), most likely on its C-terminal domain (S. A. Rice, M. C. Long, V. Lam, C. A. Spencer, J. Virol. 68:988-1001, 1994). This phosphorylation modification generates a novel form of the large subunit which we have designed IIi. In this study, we examine roles that HSV-1 gene products play in this process. An HSV-1 mutant defective in the immediate-early transcriptional activator protein ICP4 is able to efficiently induce IIi. Viruses having mutations in the genes for the ICP0, ICP6, or ICP27 proteins are also competent for IIi formation. In contrast, 22/n199, an HSV-1 mutant which contains a nonsense mutation in the gene encoding the immediate-early protein ICP22, is significantly deficient in IIi induction. This effect is seen in Vero cells, where 22/n199 grows relatively efficiently, and in human embryonic lung (HEL) cells, where 22/n199 growth in more restricted. RNAP II is recruited into viral replication compartments in 22/n199-infected cells, indicating that altered phosphorylation of RNAP II is not a prerequisite for nuclear relocalization of RNAP II. In addition, we show by nuclear run-on transcription analysis that viral gene transcription is deficient in HEL cells infected with 22/n199. Viral late gene transcription does not occur efficiently, and antisense transcription throughout the genome is diminished compared with that of the wild-type HSV-1 infection. These transcriptional effects cannot be explained by differences in viral DNA replication, since 22/n199 replicates its DNA efficiently in HEL cells. Our results demonstrated that ICP22 is necessary for virus-induced aberrant phosphorylation of RNAP II and for normal patterns of viral gene transcription in certain cell lines.

MeSH Terms
Animals Blotting, Western Cell Line Cell Nucleus/metabolism Chlorocebus aethiops DNA-Binding Proteins/metabolism Fluorescent Antibody Technique Gene Expression Regulation, Viral Genes, Viral Herpesvirus 1, Human/genetics,physiology Humans Immediate-Early Proteins/analysis,biosynthesis,metabolism Kinetics Lung Mutagenesis RNA Polymerase II/isolation & purification,metabolism Transcription, Genetic Vero Cells Viral Proteins Viral Regulatory and Accessory Proteins Virus Replication
Chemicals
DNA-Binding Proteins ICP22 protein, human herpesvirus 1 Immediate-Early Proteins Viral Proteins Viral Regulatory and Accessory Proteins EUS1 protein, Equine herpesvirus 1 RNA Polymerase II
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Rice S A
Department of Biochemistry, University of Alberta, Edmonton, Canada.
Long M C
Lam V
Schaffer P A
Spencer C A
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.69.9.5550-5559.1995
Published
1995-09-00
Pages
5550-9
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC189408
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.69.9.5550-5559.1995 PMC Full Text View journal details
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