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PMID: 1695257 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Differential regulation of endogenous and transduced beta-globin genes during infection of erythroid cells with a herpes simplex virus type 1 recombinant.

Journal of virology ·Vol. 64 ·No. 8 ·1990-08-00 ·Pages 3882-94

Smibert CA, Smiley JR

Abstract

We infected murine erythroleukemia cells with a nondefective herpes simplex virus (HSV) type 1 recombinant bearing the rabbit beta-globin gene under the control of its own promoter, in order to compare the regulation of a cellular gene residing in the viral genome to that of its active endogenous counterpart. We found that the viral globin gene was activated by HSV immediate-early polypeptides, whereas expression of the endogenous beta-globin gene was strongly suppressed: transcription was greatly inhibited, and beta-globin mRNA was rapidly degraded. Degradation of globin mRNA was induced by a component of the infecting virion and required a functional UL41 gene product. These results demonstrate that HSV products can have opposing effects on the expression of homologous genes located in the cellular and viral genomes and suggest that the preferential expression of HSV genes that occurs during infection is not achieved solely through sequence-specific differentiation between viral and cellular promoters or mRNAs.

MeSH Terms
Animals Base Sequence Cell Line Cell Nucleus/metabolism Cell Transformation, Viral Cycloheximide/pharmacology Dactinomycin/pharmacology Gene Expression Regulation Gene Expression Regulation, Viral Genes Globins/genetics Kinetics Mice Molecular Sequence Data Mutation Oligonucleotide Probes RNA/genetics,isolation & purification Rabbits Restriction Mapping Simplexvirus/genetics Transcription, Genetic/drug effects Transduction, Genetic
Chemicals
Oligonucleotide Probes Dactinomycin RNA Globins Cycloheximide
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Smibert C A
Pathology Department, McMaster University, Hamilton, Ontario, Canada.
Smiley J R
References (77)
77 references, click to expand
  1. The human beta-globin gene contains multiple regulatory regions: identification of one promoter and two downstream enhancers.
    EMBO J. 1988 Feb;7(2):377-84 PMID: 2835225
  2. A 3' enhancer is required for temporal and tissue-specific transcriptional activation of the chicken adult beta-globin gene.
    Nature. 1986 Oct 23-29;323(6090):731-4 PMID: 3022151
  3. Expression of a cellular gene cloned in herpes simplex virus: rabbit beta-globin is regulated as an early viral gene in infected fibroblasts.
    J Virol. 1987 Aug;61(8):2368-77 PMID: 3037101
  4. Position-independent, high-level expression of the human beta-globin gene in transgenic mice.
    Cell. 1987 Dec 24;51(6):975-85 PMID: 3690667
  5. Herpes simplex virus-infected cells contain a function(s) that destabilizes both host and viral mRNAs.
    Proc Natl Acad Sci U S A. 1987 Apr;84(7):1926-30 PMID: 3031658
  6. Regulation of the herpes simplex virus type 1 late (gamma 2) glycoprotein C gene: sequences between base pairs -34 to +29 control transient expression and responsiveness to transactivation by the products of the immediate early (alpha) 4 and 0 genes.
    Nucleic Acids Res. 1987 Apr 10;15(7):3097-111 PMID: 3031620
  7. Intranuclear localization of herpes simplex virus immediate-early and delayed-early proteins: evidence that ICP 4 is associated with progeny virus DNA.
    J Gen Virol. 1986 Oct;67 ( Pt 10):2163-77 PMID: 3020158
  8. Herpes simplex virus virion host shutoff function.
    J Virol. 1988 Mar;62(3):912-21 PMID: 2828686
  9. A modular system for the assay of transcription regulatory signals: the sequence TAATGARAT is required for herpes simplex virus immediate early gene activation.
    Nucleic Acids Res. 1985 Nov 11;13(21):7847-63 PMID: 2999706
  10. Herpes simplex virus regulatory elements and the immunoglobulin octamer domain bind a common factor and are both targets for virion transactivation.
    Cell. 1988 Feb 12;52(3):435-45 PMID: 2830987
  11. A complex formed between cell components and an HSV structural polypeptide binds to a viral immediate early gene regulatory DNA sequence.
    Cell. 1988 Feb 12;52(3):425-34 PMID: 2830986
  12. A specific 15-bp TATA box promoter element is required for expression of a herpes simplex virus type 1 late gene.
    Genes Dev. 1988 Jan;2(1):40-53 PMID: 2833425
  13. The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1.
    J Gen Virol. 1988 Jul;69 ( Pt 7):1531-74 PMID: 2839594
  14. Induction and repression of cellular gene transcription during herpes simplex virus infection are mediated by different viral immediate-early gene products.
    Eur J Biochem. 1988 Jun 1;174(2):443-9 PMID: 2838278
  15. An ICP6::lacZ insertional mutagen is used to demonstrate that the UL52 gene of herpes simplex virus type 1 is required for virus growth and DNA synthesis.
    J Virol. 1988 Aug;62(8):2970-7 PMID: 2839713
  16. Promoter sequence and cell type can dramatically affect the efficiency of transcriptional activation induced by herpes simplex virus type 1 and its immediate-early gene products Vmw175 and Vmw110.
    J Mol Biol. 1988 Oct 5;203(3):739-51 PMID: 2850365
  17. The human beta-globin gene contains a downstream developmental specific enhancer.
    Nucleic Acids Res. 1987 Jul 24;15(14):5739-47 PMID: 3039464
  18. A herpesvirus trans-activating protein interacts with transcription factor OTF-1 and other cellular proteins.
    Proc Natl Acad Sci U S A. 1988 Sep;85(17):6347-51 PMID: 2842768
  19. A genetic approach to promoter recognition during trans induction of viral gene expression.
    Science. 1986 Oct 3;234(4772):53-9 PMID: 3018926
  20. Isolation of cDNA clones derived from a cellular gene transcriptionally induced by herpes simplex virus.
    Nucleic Acids Res. 1986 Jul 25;14(14):5629-40 PMID: 3016658
  21. A mutant of herpes simplex virus type 1 exhibits increased stability of immediate-early (alpha) mRNAs.
    J Virol. 1987 Feb;61(2):604-6 PMID: 3027388
  22. Stages in the nuclear association of the herpes simplex virus transcriptional activator protein ICP4.
    J Virol. 1987 Feb;61(2):276-84 PMID: 3027360
  23. The control of herpes simplex virus type-1 late gene transcription: a 'TATA-box'/cap site region is sufficient for fully efficient regulated activity.
    Nucleic Acids Res. 1986 Nov 11;14(21):8247-64 PMID: 3024102
  24. Host cell proteins bind to the cis-acting site required for virion-mediated induction of herpes simplex virus 1 alpha genes.
    Proc Natl Acad Sci U S A. 1987 Jan;84(1):71-5 PMID: 3025864
  25. Transcriptional control signals of a herpes simplex virus type 1 late (gamma 2) gene lie within bases -34 to +124 relative to the 5' terminus of the mRNA.
    Mol Cell Biol. 1986 Nov;6(11):3652-66 PMID: 3025606
  26. Homologous recognition of a promoter domain common to the MSV LTR and the HSV tk gene.
    Cell. 1986 Feb 28;44(4):565-76 PMID: 3004739
  27. Analysis of DNA sequences which regulate the transcription of herpes simplex virus immediate early gene 3: DNA sequences required for enhancer-like activity and response to trans-activation by a virion polypeptide.
    Nucleic Acids Res. 1986 Jan 24;14(2):929-43 PMID: 3003700
  28. Effects of herpes simplex virus on mRNA stability.
    J Virol. 1987 Jul;61(7):2198-207 PMID: 3035220
  29. Differential stability of host mRNAs in Friend erythroleukemia cells infected with herpes simplex virus type 1.
    J Virol. 1985 Jan;53(1):1-6 PMID: 2981326
  30. Two distinct transcription factors bind to the HSV thymidine kinase promoter in vitro.
    Cell. 1985 Sep;42(2):559-72 PMID: 2992804
  31. The role of viral and cellular nuclear proteins in herpes simplex virus replication.
    Adv Virus Res. 1989;37:85-123 PMID: 2557760
  32. Control of mRNA stability by the virion host shutoff function of herpes simplex virus.
    J Virol. 1989 May;63(5):1897-906 PMID: 2539493
  33. During latency, herpes simplex virus type 1 DNA is associated with nucleosomes in a chromatin structure.
    J Virol. 1989 Feb;63(2):943-7 PMID: 2536115
  34. Transduction of the Chinese hamster ovary aprt gene by herpes simplex virus.
    J Virol. 1984 Nov;52(2):606-14 PMID: 6092682
  35. Identification of herpes simplex virus DNA sequences which encode a trans-acting polypeptide responsible for stimulation of immediate early transcription.
    J Mol Biol. 1984 Nov 25;180(1):1-19 PMID: 6096556
  36. Trans activation of transcription by herpes virus products: requirement for two HSV-1 immediate-early polypeptides for maximum activity.
    EMBO J. 1984 Dec 20;3(13):3135-41 PMID: 6098466
  37. The regulation of transcription of viral and cellular genes by herpesvirus immediate-early gene products (review).
    Anticancer Res. 1987 Jul-Aug;7(4A):589-604 PMID: 3310848
  38. Transcriptional selectivity of viral genes in mammalian cells.
    Cell. 1986 Sep 12;46(6):795-805 PMID: 3530495
  39. Degradation of cellular mRNAs induced by a virion-associated factor during herpes simplex virus infection of Vero cells.
    J Virol. 1985 Sep;55(3):601-10 PMID: 4020960
  40. Stability of globin mRNA in terminally differentiating murine erythroleukemia cells.
    Cell. 1981 Feb;23(2):509-14 PMID: 6937265
  41. Globin RNA transcription: a possible termination site and demonstration of transcriptional control correlated with altered chromatin structure.
    Cell. 1982 Jul;29(3):887-93 PMID: 7151174
  42. Herpes simplex virus types 1 and 2 induce shutoff of host protein synthesis by different mechanisms in Friend erythroleukemia cells.
    J Virol. 1983 Jan;45(1):241-50 PMID: 6296433
  43. Construction of a double-jointed herpes simplex viral DNA molecule: inverted repeats are required for segment inversion, and direct repeats promote deletions.
    Virology. 1981 Aug;113(1):345-62 PMID: 6267787
  44. Structural features of the herpes simplex virus alpha gene 4, 0, and 27 promoter-regulatory sequences which confer alpha regulation on chimeric thymidine kinase genes.
    J Virol. 1982 Dec;44(3):939-49 PMID: 6294341
  45. Generation of genetic diversity in herpes simplex virus: an antimutator phenotype maps to the DNA polymerase locus.
    Virology. 1984 Jan 15;132(1):26-37 PMID: 6320535
  46. DNA sequence elements required for regulated expression of the HSV-1 glycoprotein D gene lie within 83 bp of the RNA capsites.
    Nucleic Acids Res. 1983 Oct 11;11(19):6647-66 PMID: 6314251
  47. The effect of cycloheximide on the accumulation and stability of functional alpha-mRNA in cells infected with herpes simplex virus.
    J Gen Virol. 1983 Sep;64 (Pt 9):1955-63 PMID: 6310035
  48. Analysis of DNA sequences which regulate the transcription of a herpes simplex virus immediate early gene.
    J Virol. 1984 Jun;50(3):708-16 PMID: 6328000
  49. A detailed analysis of an HSV-1 early promoter: sequences involved in trans-activation by viral immediate-early gene products are not early-gene specific.
    Nucleic Acids Res. 1984 Apr 11;12(7):3037-56 PMID: 6326049
  50. Herpes simplex virus mutants defective in the virion-associated shutoff of host polypeptide synthesis and exhibiting abnormal synthesis of alpha (immediate early) viral polypeptides.
    J Virol. 1983 May;46(2):498-512 PMID: 6302315
  51. Characterization of the herpes simplex virion-associated factor responsible for the induction of alpha genes.
    J Virol. 1983 May;46(2):371-7 PMID: 6302308
  52. Characterization of the herpes simplex virus type 1 glycoprotein D mRNA and expression of this protein in Xenopus oocytes.
    Nucleic Acids Res. 1983 Mar 11;11(5):1507-22 PMID: 6298745
  53. Separation of sequences defining basal expression from those conferring alpha gene recognition within the regulatory domains of herpes simplex virus 1 alpha genes.
    Proc Natl Acad Sci U S A. 1984 Jul;81(13):4065-9 PMID: 6330737
  54. Analysis of transcriptional regulatory signals of the HSV thymidine kinase gene: identification of an upstream control region.
    Cell. 1981 Aug;25(2):385-98 PMID: 6269744
  55. Herpes simplex virus-induced changes in cellular and adenovirus RNA metabolism in an adenovirus type 5-transformed human cell line.
    J Virol. 1982 May;42(2):474-87 PMID: 6283168
  56. Regulation of alpha genes of herpes simplex virus: expression of chimeric genes produced by fusion of thymidine kinase with alpha gene promoters.
    Cell. 1981 May;24(2):555-65 PMID: 6263501
  57. Early and delayed shut-off of host protein synthesis in cells infected with herpes simplex virus.
    J Gen Virol. 1982 Jul;61 (Pt l):121-5 PMID: 6288847
  58. The distal transcription signals of the herpesvirus tk gene share a common hexanucleotide control sequence.
    Cell. 1984 May;37(1):253-62 PMID: 6233005
  59. Construction in vitro and rescue of a thymidine kinase-deficient deletion mutation of herpes simplex virus.
    Nature. 1980 May 29;285(5763):333-5 PMID: 6246453
  60. The structure of herpes simplex virus type 1 DNA as probed by micrococcal nuclease digestion.
    J Gen Virol. 1980 Nov;51(Pt 1):45-59 PMID: 6257837
  61. Requirement of protein synthesis for the degradation of host mRNA in Friend erythroleukemia cells infected wtih herpes simplex virus type 1.
    J Virol. 1978 Sep;27(3):619-27 PMID: 212585
  62. The sequence of the chromosomal mouse beta-globin major gene: homologies in capping, splicing and poly(A) sites.
    Cell. 1978 Dec;15(4):1125-32 PMID: 569555
  63. Erythroleukemic differentiation.
    Annu Rev Biochem. 1978;47:419-48 PMID: 354501
  64. Activation of cellular promoters during herpes virus infection of biochemically transformed cells.
    EMBO J. 1985 Aug;4(8):1973-80 PMID: 2998778
  65. Virion component of herpes simplex virus type 1 KOS interferes with early shutoff of host protein synthesis induced by herpes simplex virus type 2 186.
    J Virol. 1985 Oct;56(1):312-6 PMID: 2993660
  66. The responsiveness of human papillomavirus upstream regulatory regions to herpes simplex virus immediate early proteins.
    Virus Res. 1988 Oct;11(3):199-207 PMID: 2848381
  67. OBP100 binds remarkably degenerate octamer motifs through specific interactions with flanking sequences.
    Genes Dev. 1988 Nov;2(11):1400-13 PMID: 2850260
  68. On the control of immediate early (alpha) mRNA survival in cells infected with herpes simplex virus.
    J Gen Virol. 1988 Nov;69 ( Pt 11):2869-77 PMID: 2846763
  69. Degradation of cellular mRNA during infection by herpes simplex virus.
    Proc Natl Acad Sci U S A. 1977 Jun;74(6):2370-4 PMID: 196289
  70. The primary structure of rabbit beta-globin mRNA as determined from cloned DNA.
    Cell. 1977 Apr;10(4):571-85 PMID: 558827
  71. Random cleavage of intranuclear herpes simplex virus DNA by micrococcal nuclease.
    FEBS Lett. 1979 Apr 1;100(1):107-9 PMID: 220083
  72. The effect of herpes virus infection on mRNA in polyoma virus transformed cells.
    Virology. 1976 Dec;75(2):477-80 PMID: 188239
  73. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.
    Cell. 1977 Nov;12(3):721-32 PMID: 922889
  74. Regulation of herpesvirus macromolecular synthesis. I. Cascade regulation of the synthesis of three groups of viral proteins.
    J Virol. 1974 Jul;14(1):8-19 PMID: 4365321
  75. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  76. Regulation of cellular genes transduced by herpes simplex virus.
    J Virol. 1989 May;63(5):1929-37 PMID: 2539495
  77. The beta-globin dominant control region activates homologous and heterologous promoters in a tissue-specific manner.
    Cell. 1989 Mar 24;56(6):969-77 PMID: 2924354
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.64.8.3882-3894.1990
Published
1990-08-00
Pages
3882-94
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC249684
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.64.8.3882-3894.1990 PMC Full Text View journal details
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