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PMID: 3037101 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Expression of a cellular gene cloned in herpes simplex virus: rabbit beta-globin is regulated as an early viral gene in infected fibroblasts.

Journal of virology ·Vol. 61 ·No. 8 ·1987-08-00 ·Pages 2368-77

Smiley JR, Smibert C, Everett RD

Abstract

We constructed nondefective herpes simplex virus type 1 recombinants bearing the intact rabbit beta-globin gene inserted into the viral gene for thymidine kinase to study the expression of a cellular gene when it is present in the viral genome during lytic viral infections. The globin promoter was activated to high levels during productive infection of Vero cells, giving rise to properly spliced and processed cytoplasmic globin transcripts. Expression of globin RNA occurred with early kinetics, was not affected by blocking viral DNA replication, and was strongly inhibited by preventing viral immediate-early protein synthesis with cycloheximide. These results support the hypothesis that temporal control of herpes simplex virus early gene expression is accomplished by mechanisms that are not restricted to viral promoters. In addition, these data show that a cellular transcript can be correctly processed and can accumulate to high levels during viral infection; this indicates that the mechanisms of virally induced shutoff of host RNA accumulation and degradation of host mRNAs do not depend on sequence-specific differentiation between host and viral RNAs. These findings also suggest that herpesviruses have considerable potential as high-capacity gene transfer vectors for a variety of applications.

MeSH Terms
Animals Aphidicolin Cycloheximide/pharmacology DNA, Recombinant DNA, Viral/genetics Diterpenes/pharmacology Gene Expression Regulation/drug effects Genes, Viral Globins/genetics Promoter Regions, Genetic RNA Processing, Post-Transcriptional Rabbits Simplexvirus/genetics Time Factors Transcription, Genetic
Chemicals
DNA, Recombinant DNA, Viral Diterpenes Aphidicolin Globins Cycloheximide
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Smiley J R
Smibert C
Everett R D
References (80)
80 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.61.8.2368-2377.1987
Published
1987-08-00
Pages
2368-77
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC255648
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.61.8.2368-2377.1987 PMC Full Text View journal details
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