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PMID: 6325883 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Transactivation of a late herpes simplex virus promoter.

Molecular and cellular biology ·Vol. 4 ·No. 3 ·1984-03-00 ·Pages 544-51

Dennis D, Smiley JR

Abstract

We have asked whether the promoter for the gene encoding the major capsid protein (VP5) of herpes simplex virus functions in uninfected mouse cells. Our experimental strategy was to first fuse the VP5 promoter to the herpes simplex virus thymidine kinase (TK) structural sequence and then to use the resulting hybrid gene to transform TK- cells to TK+. The recombinant gene transferred TK at an extremely low frequency by comparison with the wild-type TK gene, and the TK transcripts present within the resulting rare transformants initiated within the TK structural gene, rather than in the vicinity of the VP5 promoter. However, after infection with herpes simplex virus, large amounts of RNA driven from the VP5 promoter accumulated. We conclude that the VP5 promoter does not function in uninfected cells but is efficiently activated by virally coded factors, most likely one or more immediate-early proteins.

MeSH Terms
Animals Base Sequence Capsid/genetics DNA Restriction Enzymes Enzyme Activation Genes Genes, Viral Kinetics L Cells/enzymology Mice Mutation Operon Plasmids Simplexvirus/genetics Thymidine Kinase/deficiency,genetics
Chemicals
Thymidine Kinase DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Dennis D
Smiley J R
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44 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.4.3.544-551.1984
Published
1984-03-00
Pages
544-51
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC368734
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.4.3.544-551.1984 PMC Full Text View journal details
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