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PMID: 6290997 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Sequence requirements for the transcription of the rabbit beta-globin gene in vivo: the -80 region.

Nucleic acids research ·Vol. 10 ·No. 16 ·1982-08-25 ·Pages 4951-71

Grosveld GC, Rosenthal A, Flavell RA

Abstract

We have performed a detailed analysis of DNA sequences in the -80 region of the rabbit beta-globin gene that are required for transcription. A variety of rabbit beta-globin gene templates deleted at various sites in the -80 region were linked to an SV40-plasmid recombinant and introduced into HeLa cells; under these conditions the rabbit beta-globin gene is expressed from its own promotor. The results show that the conserved GGCCAATCT sequence is required for efficient transcription in vivo and further identify another sequence in the region from about -81 to -96 which is also required for transcription in vivo.

MeSH Terms
Animals Base Sequence Chromosome Deletion Cloning, Molecular DNA Restriction Enzymes DNA Transposable Elements Genes Globins/genetics Mutation Rabbits Transcription, Genetic
Chemicals
DNA Transposable Elements Globins DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Grosveld G C
Rosenthal A
Flavell R A
References (21)
21 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.16.4951
Published
1982-08-25
Pages
4951-71
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320844
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/10.16.4951 PMC Full Text View journal details
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