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PMID: 8289400 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

RNA polymerase II is aberrantly phosphorylated and localized to viral replication compartments following herpes simplex virus infection.

Journal of virology ·Vol. 68 ·No. 2 ·1994-02-00 ·Pages 988-1001

Rice SA, Long MC, Lam V, Spencer CA

Abstract

During lytic infection, herpes simplex virus subverts the host cell RNA polymerase II transcription machinery to efficiently express its own genome while repressing the expression of most cellular genes. The mechanism by which RNA polymerase II is directed to the viral delayed-early and late genes is still unresolved. We report here that RNA polymerase II is preferentially localized to viral replication compartments early after infection with herpes simplex virus type 1. Concurrent with recruitment of RNA polymerase II into viral compartments is a rapid and aberrant phosphorylation of the large subunit carboxy-terminal domain (CTD). Aberrant phosphorylation of the CTD requires early viral gene expression but is not dependent on viral DNA replication or on the formation of viral replication compartments. Localization of RNA polymerase II and modifications to the CTD may be instrumental in favoring transcription of viral genes and repressing specific transcription of cellular genes.

MeSH Terms
Animals Biological Transport Cell Compartmentation Cell Nucleus/metabolism Cells, Cultured Gene Expression Regulation, Viral Herpesvirus 1, Human/enzymology,growth & development Humans Immunohistochemistry Phosphorylation Protein Processing, Post-Translational RNA Polymerase II/isolation & purification,metabolism Time Factors Transcription, Genetic Virus Replication
Chemicals
RNA Polymerase II
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Rice S A
Department of Biochemistry, University of Alberta, Edmonton, Canada.
Long M C
Lam V
Spencer C A
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.68.2.988-1001.1994
Published
1994-02-00
Pages
988-1001
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC236537
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.68.2.988-1001.1994 PMC Full Text View journal details
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