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PMID: 8396283 Published · ppublish English Journal Article

In vitro characterization of a herpes simplex virus type 1 ICP22 deletion mutant.

Virus genes ·Vol. 7 ·No. 2 ·1993-06-00 ·Pages 171-86

Poffenberger KL, Raichlen PE, Herman RC

Abstract

We report the construction of a deletion mutant (del22Z) that is unable to synthesize any detectable messenger RNA or protein products from the herpes simplex virus type 1 (HSV-1) immediate early ICP22 gene upon infection. The del22Z deletion mutant lacks all but 18 nucleotides of the ICP22 coding sequence and carries the bacterial lacZ gene at the site of the deletion. No other known open reading frames or flanking sequences were disrupted. Del22Z was able to infect Vero cells productively but was severely restricted in human and rodent cells that were permissive for the parental HSV-1(F). The yield of del22Z was not enhanced significantly, either by increasing the multiplicity of infection or by increasing the duration of the infection. There was a prolonged expression of some early gene products and a delayed appearance of some late gene products in both permissive and restrictive cells. This phenotype of cell-line restricted growth and alteration of the normal gene expression cascade maps specifically to the ICP22 coding region.

Related Genes
ICP22
MeSH Terms
Cell Line Defective Viruses/genetics,pathogenicity Escherichia coli/genetics Gene Deletion Gene Expression Genes, Viral Genetic Markers Genome, Viral Immediate-Early Proteins Mutagenesis, Site-Directed Simplexvirus/genetics,pathogenicity Transcription, Genetic Viral Proteins/genetics Viral Regulatory and Accessory Proteins Virulence/genetics
Chemicals
Genetic Markers ICP22 protein, human herpesvirus 1 IE1 protein, Human herpesvirus 1 Immediate-Early Proteins Viral Proteins Viral Regulatory and Accessory Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Poffenberger K L
Syntex Research, Palo Alto, California 94304.
Raichlen P E
Herman R C
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41 references, click to expand
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Article Info
Journal
Virus genes
Abbr.
Virus Genes
ISSN
0920-8569
DOI
10.1007/BF01702397
Published
1993-06-00
Pages
171-86
Language
English
Region
United States
NLM ID
8803967
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01702397 View journal details
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