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PMID: 3005980 Published · ppublish English Comparative Study Journal Article

Complete DNA sequence of the short repeat region in the genome of herpes simplex virus type 1.

Nucleic acids research ·Vol. 14 ·No. 4 ·1986-02-25 ·Pages 1727-45

McGeoch DJ, Dolan A, Donald S, Brauer DH

Abstract

We report the complete DNA sequence of the short repeat region in the genome of herpes simplex virus type 1, as 6633 base pairs of composition 79.5% G+C. This contains immediate early gene 3, encoding the IE175 protein, an important transcriptional activator of later virus genes. The IE175 coding region was identified as a 3894 base sequence of 81.5% G+C DNA. The base composition of this gene is thus the most extreme yet determined, and the IE175 predicted amino acid composition is correspondingly biased, most notably with an alanine content of 20.9%. Functionally important regions of the IE175 polypeptide were tentatively identified by comparison with the sequence of the homologous protein from varicella-zoster virus and from locations of ts mutations, and were correlated with properties of the amino acid sequence. Aspects of the evolution of such an extreme composition DNA sequence were discussed.

MeSH Terms
Amino Acid Sequence Base Composition Base Sequence Codon DNA, Viral/genetics Genes, Viral Repetitive Sequences, Nucleic Acid Simplexvirus/genetics Structure-Activity Relationship Vesicular stomatitis Indiana virus/genetics Viral Proteins/genetics
Chemicals
Codon DNA, Viral Viral Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
McGeoch D J
Dolan A
Donald S
Brauer D H
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54 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.4.1727
Published
1986-02-25
Pages
1727-45
Language
English
Region
England
NLM ID
0411011
PMCID
PMC339569
Subset
IM
Databases
GENBANK
L00036, L00037, M12354, M12506, X00428
External Links
PubMed source DOI: 10.1093/nar/14.4.1727 PMC Full Text View journal details
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