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PMID: 6322134 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Replication origins and a sequence involved in coordinate induction of the immediate-early gene family are conserved in an intergenic region of herpes simplex virus.

Nucleic acids research ·Vol. 12 ·No. 4 ·1984-02-24 ·Pages 2061-79

Whitton JL, Clements JB

Abstract

We have determined the structure of the 5' portion of herpes simplex virus type 2 (HSF-2) immediate-early (IE) mRNA-3 and have obtained the DNA sequence specifying the N terminus of its encoded polypeptide, Vmw182, its untranslated leader and the intergenic region between IEmRNAs-3 & 4/5. Comparison of the HSV-2 intergenic sequences with the HSV-1 equivalent region identifies several conserved regions: (1) an AT-rich element with core consensus TAATGARAT which is likely to be the 'activator' sequence through which coordinate induction of the IE gene family is mediated. (2) GC-rich and GA-rich tracts, found in a wide variety of eukaryotic promoters, which vary in position and orientation between HSV-2 and HSV-1 and which represent modulators of transcription. (3) TATA homologies present 15-25 base pairs (bp) upstream of mRNA 5' termini. (4) a 137bp direct repeat in HSV-2 which contains sequence almost identical to the HSV-1 replication origin.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cell Line Cloning, Molecular Cricetinae DNA Replication DNA Restriction Enzymes Genes Genes, Viral Kidney Molecular Weight Operon RNA, Messenger/genetics Simplexvirus/genetics Transcription, Genetic Viral Proteins/genetics
Chemicals
RNA, Messenger Viral Proteins DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Whitton J L
Clements J B
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47 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.4.2061
Published
1984-02-24
Pages
2061-79
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318641
Subset
IM
Databases
GENBANK
X00416
External Links
PubMed source DOI: 10.1093/nar/12.4.2061 PMC Full Text View journal details
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