Solid phase synthesis of polynucleotides. VI. Further studies on polystyrene copolymers for the solid support.
A simple solid phase method for the synthesis of oligodeoxyribonucleotides has been developed using the phosphotriester approach. Mononucleotide coupling units are sequentially added to the polystyrene copolymer with 1% divinylbenzene and two kinds of oligonucleotides, d(CACGACCCCTCCACGT) and d(AACTGGTATTACTGGGCG), are synthesized in a relatively high yield. One cycle of the mononucleotide addition is about 70 minutes, and this method is particularly suitable for the automation of the synthesis upon availability of an automatic synthesizer.
13 references, click to expand
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DNA sequence analysis: a general, simple and rapid method for sequencing large oligodeoxyribonucleotide fragments by mapping.Nucleic Acids Res. 1974 Mar;1(3):331-53 PMID: 10793670
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Letter: Phosphite coupling procedure for generating internucleotide links.J Am Chem Soc. 1975 May 28;97(11):3278-9 PMID: 1133350
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Arylsulfonyltetrazoles, new coupling reagents and further improvements in the triester method for the synthesis of deoxyribooligonucleotides.Nucleic Acids Res. 1977 Feb;4(2):353-71 PMID: 190591
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Rapid synthesis of oligodeoxyribonucleotides. IV. Improved solid phase synthesis of oligodeoxyribonucleotides through phosphotriester intermediates.Nucleic Acids Res. 1980 Mar 11;8(5):1081-96 PMID: 7443540
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Solid phase phosphotriester synthesis of large oligodeoxyribonucleotides on a polyamide support.Nucleic Acids Res. 1980 Nov 25;8(22):5193-205 PMID: 7465412
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Simplications in the synthesis of short oligonucleotide blocks.Nucleic Acids Res. 1980 Nov 25;8(22):5461-71 PMID: 7465417
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Solid-phase synthesis of polynucleotides. III. Synthesis of polynucleotides with defined sequences by the block coupling phosphotriester method.Nucleic Acids Res. 1980 Nov 25;8(22):5491-505 PMID: 7465419
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Solid-phase synthesis of polynucleotides. IV. Usage of polystyrene resins for the synthesis of polydeoxyribonucleotides by the phosphostriester method.Nucleic Acids Res. 1980 Nov 25;8(22):5507-17 PMID: 7465420
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Rapid synthesis of oligodeoxyribonucleotides VI. Efficient, mechanised synthesis of heptadecadeoxyribonucleotides by an improved solid phase phosphotriester route.Nucleic Acids Res. 1981 Apr 10;9(7):1691-706 PMID: 7232224
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Synthesis of oligonucleotides on cellulose. Part II: Design and synthetic strategy to the synthesis of 22 oligodeoxynucleotides coding for gastric inhibitory polypeptide (GIP).Nucleic Acids Symp Ser. 1980;(7):225-32 PMID: 6265873
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Rapid synthesis of oligodeoxyribonucleotides. V. Further studies in solid phase synthesis of oligodeoxyribonucleotides through phosphotriester intermediates.Nucleic Acids Symp Ser. 1980;(7):243-57 PMID: 7255172
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Total synthesis of a human leukocyte interferon gene.Nature. 1981 Aug 20;292(5825):756-62 PMID: 6167861
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Synthesis of oligodeoxyribonucleotides on silica gel support.Nucleic Acids Res. 1981 Jun 25;9(12):2807-17 PMID: 6269061
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