Rapid synthesis of oligodeoxyribonucleotides. IV. Improved solid phase synthesis of oligodeoxyribonucleotides through phosphotriester intermediates.

Nucleic acids research ·Vol. 8 ·No. 5 ·1980-03-11 ·Pages 1081-96

Gait MJ, Singh M, Sheppard RC, Edge MD, Greene AR, Heathcliffe GR, Atkinson TC, Newton CR, Markham AF

Abstract

A phosphotriester solid phase method on a polyamide support has been used to prepare oligodeoxyribonucleotides up to 12 units long. Compared to solid phase phosphodiester synthesis the new methodology is quicker, more flexible and gives 10-60-fold better overall yields.

MeSH Terms

Chemical Phenomena Chemistry Methods Oligodeoxyribonucleotides/chemical synthesis Oligonucleotides/chemical synthesis Organophosphates Resins, Plant

Chemicals

Oligodeoxyribonucleotides Oligonucleotides Organophosphates Resins, Plant

Authors & Affiliations

9 authors, click to expand affiliations / ORCID

Gait M J

Singh M

Sheppard R C

Edge M D

Greene A R

Heathcliffe G R

Atkinson T C

Newton C R

Markham A F

References (16)

16 references, click to expand

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Synthesis of human insulin gene. Part I. Development of reversed-phase chromatography in the modified triester method. Its application in the rapid and efficient synthesis of eight deoxyribooligonucleotides fragments constituting human insulin A DNA.
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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/8.5.1081

Published

1980-03-11

Pages

1081-96

Language

English

Region

England

NLM ID

0411011

PMCID

PMC323974

Subset

External Links

PubMed source DOI: 10.1093/nar/8.5.1081 PMC Full Text View journal details

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Rapid synthesis of oligodeoxyribonucleotides. IV. Improved solid phase synthesis of oligodeoxyribonucleotides through phosphotriester intermediates.

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