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PMID: 866189 Published · ppublish English Journal Article

Rapid synthesis of oligodeoxyribonucleotides: a new solid-phase method.

Nucleic acids research ·Vol. 4 ·No. 4 ·1977-04-00 ·Pages 1135-58

Gait MJ, Sheppard RC

Abstract

A method is described that makes use of a new polyamide resin for the rapid synthesis of short oligodeoxyribonucleotides. The method is illustrated by the preparation of two heptadeoxyribonucleotides, d(pT6-C) and d(pC-A-G-T-G-A-T) using a phosphodiester approach. A further development involved use of phenyl isocyanate as an in situ drying agent, which obviated the need for solvent co-evaporation prior tothe internucleotidic coupling steps. Improved fractionation of thymidyl oligonucleotides was obtained by use of a new microparticulate, silica-based anion-exchanger.

MeSH Terms
Amides Methods Nitriles Oligodeoxyribonucleotides/chemical synthesis,isolation & purification Oligonucleotides/chemical synthesis Resins, Plant
Chemicals
Amides Nitriles Oligodeoxyribonucleotides Oligonucleotides Resins, Plant
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Gait M J
Sheppard R C
References (23)
23 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/4.4.1135
Published
1977-04-00
Pages
1135-58
Language
English
Region
England
NLM ID
0411011
PMCID
PMC342510
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/4.4.1135 PMC Full Text View journal details
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