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PMID: 7050755 Published · ppublish English Comparative Study Journal Article

Monoclonal antibodies as neural cell surface markers.

Neurochemical research ·Vol. 7 ·No. 3 ·1982-03-00 ·Pages 349-62

Langley OK, Ghandour MS, Gombos G, Hirn M, Goridis C

Abstract

A comparison is made of the immunohistochemistry at the ultrastructural level of three monoclonal antibodies directed against surface components of DNS cells. Hybridomas secreting these antibodies were obtained from two cell fusions of a rat myeloma cell line and immune splenocytes derived from rats immunized either with primary mouse brain cultured cells or membrane components. In cultures one antibody, anti-BSP2 (Brain Surface Protein-2), was preferentially directed against neurons while another, anti-BSP-3 (Brain Surface Protein-3), preferentially labeled astrocytes. In mouse cerebellar sections, both labeled the surface of Purkinje cells, granule cells and astrocytes. In addition a cytoplasm localization was apparent in granule cells and astrocytes. Another antibody anti-MESA-1 (Mouse Endothelial Surface Antigen-1) reacted exclusively with the surface of endothelial cells lining blood vessels. These data are discussed with reference to the biochemical nature of the corresponding antigens and to known glycoproteins of neural cell membranes.

MeSH Terms
Animals Antibodies, Monoclonal/immunology Antigens, Surface/immunology Astrocytes/immunology Cell Membrane/immunology Cells, Cultured Cerebellum/cytology Endothelium/immunology Glycoproteins/immunology Hybridomas/immunology Immunologic Techniques Male Mice Mice, Inbred C57BL Nerve Tissue Proteins/immunology Neurons/immunology Purkinje Cells/immunology Rats
Chemicals
Antibodies, Monoclonal Antigens, Surface Glycoproteins Nerve Tissue Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Langley O K
Ghandour M S
Gombos G
Hirn M
Goridis C
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42 references, click to expand
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Article Info
Journal
Neurochemical research
Abbr.
Neurochem Res
ISSN
0364-3190
DOI
10.1007/BF00965646
Published
1982-03-00
Pages
349-62
Language
English
Region
United States
NLM ID
7613461
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00965646 View journal details
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