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PMID: 168877 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Subcellular localization of pulmonary antiotensin-converting enzyme (kininase II).

The Biochemical journal ·Vol. 146 ·No. 2 ·1975-02-00 ·Pages 497-9

Ryan JW, Ryan US, Schultz DR, Whitaker C, Chung A

Abstract

Goat antibodies to pig lung angiotensin-converting enzyme (kininase II) were conjugated to microperoxidase. Rat lung tissue, previously incubated with non-immune goat serum, was incubated with the antibody-microperoxidase conjugate and then with H2O2 and 3,3-diaminobenzidine. Electron microscopy revealed reaction product on the plasma membrane and caveolae of endothelial cells, especially those of capillaries and venules. These results support the hypothesis that angiotensin I and bradykinin are metabolized by enzymes on the luminal surface of pulmonary endothelial cells.

MeSH Terms
Angiotensin II/analysis Animals Antibodies Bradykinin/analysis Cell Membrane/enzymology,ultrastructure Endothelium/ultrastructure Female Goats/immunology Lung/enzymology Microscopy, Electron Peptidyl-Dipeptidase A/analysis Peroxidases
Chemicals
Antibodies Angiotensin II Peroxidases Peptidyl-Dipeptidase A Bradykinin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Ryan J W
Ryan U S
Schultz D R
Whitaker C
Chung A
References (11)
11 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1460497
Published
1975-02-00
Pages
497-9
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1165329
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1460497 PMC Full Text View journal details
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