Carbonic anhydrase: an ultrastructural study in rat cerebellum.

The Histochemical journal ·Vol. 12 ·No. 4 ·1980-07-00 ·Pages 473-83

Langley OK, Ghandour MS, Vincendon G, Gombos G

Abstract

The cellular and intracellular distribution of carbonic anhydrase isozyme II in rat cerebellum has been investigated with the electron microscope by the indirect antibody immunohistochemical technique. Unequivocal evidence is presented supporting the view that this enzyme is exclusively localized in oligodendrocytes. Myelin does not appear to contain detectable amounts of carbonic anhydrase though it is present in oligodendrocyte processes and in the layer of oligodendrocyte cytoplasm frequently seen to coat the external surface of myelinated fibres. The immune precipitate is found to be confined to the cytosol and the cytosolic surfaces of intracellular membranes. The data are discussed in relation to the possible function of the enzyme and the role of oligodendrocytes in the central nervous system.

MeSH Terms

Animals Carbonic Anhydrases/metabolism Cerebellum/enzymology,ultrastructure Isoenzymes/metabolism Myelin Sheath/enzymology Oligodendroglia/enzymology Rats

Chemicals

Isoenzymes Carbonic Anhydrases

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Langley O K

Ghandour M S

Vincendon G

Gombos G

References (25)

25 references, click to expand

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Article Info

Journal

The Histochemical journal

Abbr.

Histochem J

ISSN

0018-2214

DOI

10.1007/BF01011962

Published

1980-07-00

Pages

473-83

Language

English

Region

Netherlands

NLM ID

0163161

Subset

External Links

PubMed source DOI: 10.1007/BF01011962

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Carbonic anhydrase: an ultrastructural study in rat cerebellum.

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