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PMID: 5276293 Published · ppublish English Journal Article

Rate of somatic mutation in immunoglobulin production by mouse myeloma cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 68 ·No. 1 ·1971-01-00 ·Pages 219-23

Coffino P, Scharff MD

Abstract

Cultures of mouse myeloma cells which secrete both heavy (H) and light (L) immunoglobulin chains were cloned in soft agar. Variants that synthesized neither chain, or only light chains, were detected by overlaying the growing colonies with antiserum specific for the individual immunoglobulin chains. The rate of conversion of heavy plus light chain producers to light chain producers was 1.1 x 10(-3) per cell per generation, as determined by fluctuation analysis.

MeSH Terms
Animals Carbon Isotopes Clone Cells/metabolism Culture Techniques Immunogenetics Immunoglobulin G/biosynthesis Immunoglobulins/biosynthesis,metabolism Leucine/metabolism Mice Mutation Peptide Biosynthesis Plasmacytoma/metabolism Threonine/metabolism Valine/metabolism
Chemicals
Carbon Isotopes Immunoglobulin G Immunoglobulins Threonine Leucine Valine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Coffino P
Scharff M D
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23 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.68.1.219
Published
1971-01-00
Pages
219-23
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC391199
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.68.1.219 PMC Full Text View journal details
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