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PMID: 6572383 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Antibodies of predetermined specificity detect two retroviral oncogene products and inhibit their kinase activities.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 5 ·1983-03-00 ·Pages 1246-50

Sen S, Houghten RA, Sherr CJ, Sen A

Abstract

Oligopeptides predicted from the nucleotide sequence of the oncogene v-fes of feline sarcoma virus (FeSV) were synthesized chemically and used to generate specific antibodies. Antisera against a 12-amino-acid-long oligopeptide (12-mer) located 42 residues from the carboxyl terminus of the v-fes coding sequence efficiently recognized the transforming proteins encoded by Snyder-Theilen (ST) and Gardner-Arnstein (GA) strains of FeSV. This 12-mer also contains 10 amino acid residues homologous in order and position to those predicted from the nucleotide sequence of the oncogene v-fps of avian Fujinami sarcoma virus (FSV). The anti-12-mer immunoprecipitated the FSV-specific transforming protein molecules from FSV-transformed cells. Binding of these antipeptide antibody molecules to the v-fes and the v-fps gene products inhibited their associated tyrosine-specific protein kinase (EC 2.7.1.37) activities. The ability to generate such site-specific antisera to the products of related oncogenes will be valuable in the molecular characterization of retroviral transforming proteins and their normal cellular homologs.

MeSH Terms
Antibody Specificity Base Sequence Cell Transformation, Neoplastic Oncogenes Peptides/chemical synthesis Protein Kinase Inhibitors Retroviridae/genetics
Chemicals
Peptides Protein Kinase Inhibitors
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Sen S
Houghten R A
Sherr C J
Sen A
References (38)
38 references, click to expand
  1. Color test for detection of free terminal amino groups in the solid-phase synthesis of peptides.
    Anal Biochem. 1970 Apr;34(2):595-8 PMID: 5443684
  2. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  3. The monitoring of reactions in solid-phase peptide synthesis with picric acid.
    Anal Chim Acta. 1972 Jan;58(1):248-9 PMID: 5057745
  4. Protection of tryptophan with the formyl group in peptide synthesis.
    J Org Chem. 1973 Jul 27;38(15):2594-7 PMID: 4730674
  5. Restriction endonuclease mapping of unintegrated proviral DNA of Snyder-Theilen feline sarcoma virus: localization of sarcoma-specific sequences.
    J Virol. 1979 Dec;32(3):860-75 PMID: 229270
  6. The nonstructural components of polyproteins encoded by replication-defective mammalian transforming retroviruses are phosphorylated and have associated protein kinase activity.
    Virology. 1980 Feb;101(1):185-97 PMID: 6243819
  7. Biochemical and immunological characterization of polyproteins coded for by the McDonough, Gardner-Arnstein, and Snyder-Theilen strains of feline sarcoma virus.
    J Virol. 1980 Jan;33(1):196-207 PMID: 6154148
  8. Molecular cloning of Snyder-Theilen feline leukemia and sarcoma viruses: comparative studies of feline sarcoma virus with its natural helper virus and with Moloney murine sarcoma virus.
    J Virol. 1980 Apr;34(1):200-12 PMID: 6246254
  9. Identification of phosphotyrosine as a product of epidermal growth factor-activated protein kinase in A-431 cell membranes.
    J Biol Chem. 1980 Sep 25;255(18):8363-5 PMID: 6157683
  10. Three independent isolates of feline sarcoma virus code for three distinct gag-x polyproteins.
    J Virol. 1980 Jul;35(1):259-64 PMID: 6251274
  11. Chemical synthesis of a polypeptide predicted from nucleotide sequence allows detection of a new retroviral gene product.
    Nature. 1980 Oct 30;287(5785):801-5 PMID: 6159543
  12. Molecular dissection of Rauscher virus gp70 by using monoclonal antibodies: localization of acquired sequences of related envelope gene recombinants.
    Proc Natl Acad Sci U S A. 1980 Aug;77(8):4524-8 PMID: 6933501
  13. Origin and functional properties of the major gene product of the Snyder-Theilen strain of feline sarcoma virus.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5158-62 PMID: 6254060
  14. Antibodies specific for the carboxy- and amino-terminal regions of simian virus 40 large tumor antigen.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5197-200 PMID: 6254066
  15. Nucleotide sequence analysis of the transforming region and large terminal redundancies of Moloney murine sarcoma virus.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5234-8 PMID: 6254069
  16. Nucleotide sequence and formation of the transforming gene of a mouse sarcoma virus.
    Nature. 1981 Jan 22;289(5795):258-62 PMID: 6256659
  17. Human beta-endorphin: synthesis and characterization of analogs iodinated and tritiated at tyrosine residues 1 and 27.
    Int J Pept Protein Res. 1980 Oct;16(4):311-20 PMID: 7461911
  18. Transforming proteins of some feline and avian sarcoma viruses are related structurally and functionally.
    Cell. 1981 Apr;24(1):145-53 PMID: 6263483
  19. Snyder-Theilen feline sarcoma virus P85 contains a single phosphotyrosine acceptor site recognized by its associated protein kinase.
    J Virol. 1981 Jun;38(3):886-94 PMID: 6165840
  20. Homologous tyrosine phosphorylation sites in transformation-specific gene products of distinct avian sarcoma viruses.
    Nature. 1981 Jun 25;291(5817):675-7 PMID: 6264320
  21. Chemically synthesized peptides predicted from the nucleotide sequence of the hepatitis B virus genome elicit antibodies reactive with the native envelope protein of Dane particles.
    Proc Natl Acad Sci U S A. 1981 Jun;78(6):3403-7 PMID: 6167985
  22. Recombinant bacteriophages containing the integrated transforming provirus of Gardner--Arnstein feline sarcoma virus.
    Proc Natl Acad Sci U S A. 1981 Jul;78(7):4036-40 PMID: 6270655
  23. Stimulation of tyrosine-specific phosphorylation by platelet-derived growth factor.
    Nature. 1982 Feb 4;295(5848):419-20 PMID: 6173766
  24. Antibodies against the chemically synthesized genome-linked protein of poliovirus react with native virus-specific proteins.
    Cell. 1982 Feb;28(2):395-404 PMID: 6174242
  25. Synthetic peptide fragment of src gene product inhibits the src protein kinase and crossreacts immunologically with avian onc kinases and cellular phosphoproteins.
    Proc Natl Acad Sci U S A. 1981 Dec;78(12):7412-6 PMID: 6801651
  26. Analysis of the sequence of amino acids surrounding sites of tyrosine phosphorylation.
    Proc Natl Acad Sci U S A. 1982 Feb;79(4):973-7 PMID: 6280176
  27. Avian sarcoma virus Y73 genome sequence and structural similarity of its transforming gene product to that of Rous sarcoma virus.
    Nature. 1982 May 20;297(5863):205-8 PMID: 6281656
  28. Oncogenes.
    Sci Am. 1982 Mar;246(3):80-92 PMID: 6281879
  29. Form and function of retroviral proviruses.
    Science. 1982 May 21;216(4548):812-20 PMID: 6177038
  30. A cellular protein is immunologically crossreactive with and functionally homologous to the Fujinami sarcoma virus transforming protein.
    Cell. 1982 Apr;28(4):897-906 PMID: 7094017
  31. Insulin stimulates tyrosine phosphorylation of the insulin receptor in a cell-free system.
    Nature. 1982 Aug 12;298(5875):667-9 PMID: 6178977
  32. Nucleotide sequence of the transforming gene of simian sarcoma virus.
    Proc Natl Acad Sci U S A. 1982 May;79(10):3179-82 PMID: 6285340
  33. Detection of a transforming gene product in cells transformed by Moloney murine sarcoma virus.
    Cell. 1982 Jun;29(2):417-26 PMID: 6288258
  34. Tapping the immunological repertoire to produce antibodies of predetermined specificity.
    Nature. 1982 Oct 14;299(5884):593-6 PMID: 6181415
  35. Nucleotide sequences of feline retroviral oncogenes (v-fes) provide evidence for a family of tyrosine-specific protein kinase genes.
    Cell. 1982 Oct;30(3):775-85 PMID: 6183005
  36. Nucleotide sequence of Fujinami sarcoma virus: evolutionary relationship of its transforming gene with transforming genes of other sarcoma viruses.
    Cell. 1982 Oct;30(3):787-95 PMID: 6291784
  37. Monoclonal antibodies specific to transforming polyproteins encoded by independent isolates of feline sarcoma virus.
    J Virol. 1982 Sep;43(3):896-904 PMID: 6183442
  38. DNA sequence of the viral and cellular src gene of chickens. 1. Complete nucleotide sequence of an EcoRI fragment of recovered avian sarcoma virus which codes for gp37 and pp60src.
    J Virol. 1982 Oct;44(1):1-11 PMID: 6292477
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.5.1246
Published
1983-03-00
Pages
1246-50
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC393572
Subset
IM
Grants
NCI NIH HHS · CA25803 · United States
External Links
PubMed source DOI: 10.1073/pnas.80.5.1246 PMC Full Text View journal details
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