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PMID: 6251274 Published · ppublish English Comparative Study Journal Article

Three independent isolates of feline sarcoma virus code for three distinct gag-x polyproteins.

Journal of virology ·Vol. 35 ·No. 1 ·1980-07-00 ·Pages 259-64

Ruscetti SK, Turek LP, Sherr CJ

Abstract

Cells nonproductively transformed by the Snyder-Theilen, Gardner-Arnstein, and McDonough strains of feline sarcoma virus synthesize gag-x polyproteins of 78,000, 100,000, and 180,000 daltons, respectively. These feline sarcoma virus-coded products were precipitated by antisera to polypeptides encoded by the gag gene of feline leukemia virus and by rat antisera raised to feline sarcoma virus-transformed rat tumor cells. Precipitation with rat antisera absorbed with feline leukemia virus showed that the x-portions of the three gag-x proteins were each antigenically distinct, suggesting that the src genes of the three independent isolates are not identical. Anti-x sera did not precipitate products from radiolabeled cat lymphoid tumor cells (FL74) and therefore lacked reactivity to the feline leukemia virus-induced tumor-specific antigen, FOCMA.

MeSH Terms
Animals Antigens, Viral/immunology Cats Cell Transformation, Viral Gene Products, gag Genes, Viral Helper Viruses Immunoelectrophoresis Rats Recombination, Genetic Retroviridae/genetics Sarcoma Viruses, Feline/genetics Viral Proteins/biosynthesis,genetics,immunology
Chemicals
Antigens, Viral Gene Products, gag Viral Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ruscetti S K
Turek L P
Sherr C J
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27 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.35.1.259-264.1980
Published
1980-07-00
Pages
259-64
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC288804
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.35.1.259-264.1980 PMC Full Text View journal details
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