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PMID: 6477530 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A transient increase in diacylglycerols is associated with the action of vasopressin on hepatocytes.

The Biochemical journal ·Vol. 222 ·No. 2 ·1984-09-01 ·Pages 535-40

Hughes BP, Rye KA, Pickford LB, Barritt GJ, Chalmers AH

Abstract

Vasopressin induced a transient increase of 50% in the total concentration of diacylglycerols (determined by g.l.c.) in isolated hepatocytes. The increase was maximal at 0.25 min, and the concentration of diacylglycerols in cells treated with vasopressin had returned to the basal value by 4 min. No change in the concentration of diacylglycerols was observed after the treatment of cells with glucagon. The dependency of this effect on the concentration of vasopressin was similar to that of the effect of the hormone on 45Ca2+ efflux measured at 0.1 mM extracellular Ca2+. Vasopressin increased the proportion of arachidonic acid and stearic acid and decreased the proportion of oleic acid present in the diacylglycerols. In hepatocytes prelabelled with [14C]arachidonic acid, vasopressin increased the amount of [14C]diacylglycerol. The effects of vasopressin on the total concentration of diacylglycerols and [14C]diacylglycerol were mimicked by an exogenous phospholipid phosphodiesterase (phospholipase C) from Clostridium perfringens. The results are consistent with the conclusion that the transient increase in diacylglycerols induced by vasopressin is caused by the rapid hydrolysis of both the phosphoinositides and one or more other phospholipids.

MeSH Terms
Animals Arginine Vasopressin/pharmacology Chromatography, Gas Diglycerides/metabolism Dose-Response Relationship, Drug Fatty Acids/analysis Glycerides/metabolism In Vitro Techniques Liver/cytology,drug effects,metabolism Male Rats Time Factors
Chemicals
Diglycerides Fatty Acids Glycerides Arginine Vasopressin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Hughes B P
Rye K A
Pickford L B
Barritt G J
Chalmers A H
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42 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2220535
Published
1984-09-01
Pages
535-40
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1144208
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2220535 PMC Full Text View journal details
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