Home LiteratureArticle Details
PMID: 6462909 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

RNA polymerase activity and template activity of chromatin after butyrate induced hyperacetylation of histones in Physarum.

Nucleic acids research ·Vol. 12 ·No. 13 ·1984-07-11 ·Pages 5405-17

Loidl P, Loidl A, Puschendorf B, Gröbner P

Abstract

We have studied the effect of sodium-n-butyrate on endogenous RNA polymerase in Physarum polycephalum. 1 mM butyrate strongly reduces RNA polymerase activity measured in isolated nuclei or chromatin; both RNA polymerase A as well as the alpha-amanitin sensitive RNA polymerase B are equally affected. Despite a concomitant hyperacetylation of histone H4 the template activity of chromatin, as analyzed by in vitro transcription of the chromatin with exogenous RNA polymerase from E. coli or RNA polymerase II from wheat germ, remains unaltered as compared to untreated control chromatin, indicating that there is no positive correlation between histone acetylation and template activity of chromatin for transcription in this organism. The results further indicate, that butyrate acts primarily as a quick but reversible inhibitor of protein synthesis in Physarum; the fast decrease of endogenous RNA polymerase activity after butyrate treatment is due to inhibition of enzyme synthesis rather than inactivation of other factors necessary for transcription.

MeSH Terms
Acetylation Amanitins/pharmacology Butyrates/pharmacology Butyric Acid Chromatin/drug effects,physiology DNA-Directed RNA Polymerases/metabolism Histones/metabolism Kinetics Physarum/drug effects,enzymology,metabolism Templates, Genetic Transcription, Genetic
Chemicals
Amanitins Butyrates Chromatin Histones Butyric Acid DNA-Directed RNA Polymerases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Loidl P
Loidl A
Puschendorf B
Gröbner P
References (42)
42 references, click to expand
  1. Activation of chromatin by acetylation of histone side chains.
    Proc Natl Acad Sci U S A. 1976 Nov;73(11):3937-41 PMID: 1069278
  2. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.
    Biochem J. 1956 Feb;62(2):315-23 PMID: 13293190
  3. Isolation of the nuclear histones from the Myxomycete, Physarum polycephalum.
    Arch Biochem Biophys. 1969 Nov;134(2):577-89 PMID: 5389721
  4. Changes in histone acetylation and RNA synthesis in the spleen of polycythemic mouse after erythropoietin injection.
    Biochim Biophys Acta. 1969 Dec 16;195(2):396-400 PMID: 5366928
  5. Isolation and DNA content of nuclei of Physarum polycephalum.
    Exp Cell Res. 1971 Jun;66(2):305-16 PMID: 4997359
  6. Separation and analysis of histone subfractions differing in their degree of acetylation: some correlations with genetic activity in development.
    Arch Biochem Biophys. 1972 May;150(1):44-56 PMID: 5028081
  7. Studies on histone fraction F2A1 in macro- and micronuclei of Tetrahymena pyriformis.
    J Cell Biol. 1973 Jun;57(3):773-81 PMID: 4633445
  8. Changes in histone acetyl content and in nuclear non-histone protein composition of avian erythroid cells at different stages of maturation.
    J Biol Chem. 1974 Nov 25;249(22):7358-68 PMID: 4436314
  9. Chromosomal subunits in active genes have an altered conformation.
    Science. 1976 Sep 3;193(4256):848-56 PMID: 948749
  10. Structure of chromatin containing extensively acetylated H3 and H4.
    Cell. 1978 Apr;13(4):691-9 PMID: 657272
  11. Butyrate suppression of histone deacetylation leads to accumulation of multiacetylated forms of histones H3 and H4 and increased DNase I sensitivity of the associated DNA sequences.
    Proc Natl Acad Sci U S A. 1978 May;75(5):2239-43 PMID: 276864
  12. DNAse I preferentially digests chromatin containing hyperacetylated histones.
    Biochem Biophys Res Commun. 1978 Jun 29;82(4):1346-53 PMID: 697799
  13. Resolution of histones by polyacrylamide gel electrophoresis in presence of nonionic detergents.
    Methods Cell Biol. 1978;17:223-33 PMID: 703614
  14. Effect of histone acetylation on structure and in vitro transcription of chromatin.
    Nucleic Acids Res. 1978 Oct;5(10):3523-47 PMID: 724494
  15. Increase in initiation sites for chromatin directed RNA synthesis by acetylation of chromosomal proteins.
    Biochem Biophys Res Commun. 1978 Sep 14;84(1):110-6 PMID: 365177
  16. Structure of the chromatosome, a chromatin particle containing 160 base pairs of DNA and all the histones.
    Biochemistry. 1978 Dec 12;17(25):5524-31 PMID: 728412
  17. Yeast chromatin is uniformly digested by DNase-I.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4285-8 PMID: 388438
  18. The gross level of in vitro RNA synthesis in HeLa nuclei is unaltered by histone hyperacetylation.
    Biochem Biophys Res Commun. 1979 Oct 12;90(3):917-24 PMID: 508354
  19. Patterns of nucleic acid synthesis in Physarum polycephalum.
    Prog Nucleic Acid Res Mol Biol. 1979;23:53-104 PMID: 95053
  20. Acetylation of histone H4 and its role in chromatin structure and function.
    Nature. 1980 Sep 4;287(5777):76-9 PMID: 7412879
  21. In vitro transcription of chromatin containing histones hyperacetylated in vivo.
    Nucleic Acids Res. 1980 Sep 25;8(18):4201-19 PMID: 7001364
  22. Manifold effects of sodium butyrate on nuclear function. Selective and reversible inhibition of phosphorylation of histones H1 and H2A and impaired methylation of lysine and arginine residues in nuclear protein fractions.
    J Biol Chem. 1981 Sep 25;256(18):9612-21 PMID: 6270094
  23. Sodium butyrate blocks HeLa cells preferentially in early G1 phase of the cell cycle.
    J Cell Sci. 1981 Oct;51:163-71 PMID: 7320081
  24. Effects of sodium butyrate, a new pharmacological agent, on cells in culture.
    Mol Cell Biochem. 1982 Feb 5;42(2):65-82 PMID: 6174854
  25. Cytotoxic effects of butyrate and other 'differentiation inducers' on immature lymphoid cells.
    Biochem Biophys Res Commun. 1982 Feb 26;104(4):1202-8 PMID: 6176240
  26. Hyperacetylation of histone H4 correlates with the terminal, transcriptionally inactive stages of spermatogenesis in rainbow trout.
    Dev Biol. 1982 Oct;93(2):404-15 PMID: 7141104
  27. Effects of histone hyperacetylation and hypoacetylation on RNA synthesis in HTC cells.
    J Biol Chem. 1982 Nov 25;257(22):13433-40 PMID: 7142157
  28. Histone H4 hyperacetylation and rapid turnover of its acetyl groups in transcriptionally inactive rooster testis spermatids.
    Nucleic Acids Res. 1982 Dec 20;10(24):8049-59 PMID: 7162988
  29. Response of the dTMP-synthesizing enzymes to differentiation processes in Physarum polycephalum.
    Exp Cell Res. 1983 Apr 1;144(2):385-91 PMID: 6840218
  30. Cell-cycle-dependent effects of sodium-n-butyrate in Physarum polycephalum.
    J Cell Sci. 1982 Dec;58:303-11 PMID: 7183689
  31. Histone hyperacetylation has little effect on the higher order folding of chromatin.
    Nucleic Acids Res. 1983 Jun 25;11(12):4065-75 PMID: 6866766
  32. Transcribing chromatin is not preferentially enriched with acetylated histones.
    FEBS Lett. 1983 Jul 25;158(2):281-4 PMID: 6873281
  33. Lack of correlation between histone H4 acetylation and transcription during the Physarum cell cycle.
    Nature. 1983 Sep 29-Oct 5;305(5933):446-8 PMID: 6621695
  34. Chromatin structure of hsp 70 genes, activated by heat shock: selective removal of histones from the coding region and their absence from the 5' region.
    Cell. 1984 Feb;36(2):423-31 PMID: 6420075
  35. Butyrate reversibly arrests the proliferation of normal and Rous sarcoma virus-infected chicken heart mesenchymal cells.
    Life Sci. 1984 Feb 20;34(8):803-8 PMID: 6321872
  36. Associated effects of sodium butyrate on histone acetylation and estrogen receptor in the human breast cancer cell line MCF-7.
    Biochem Biophys Res Commun. 1984 Feb 29;119(1):132-8 PMID: 6704117
  37. Histone modification in early and late Drosophila embryos.
    Biochem J. 1984 Mar 1;218(2):321-9 PMID: 6424659
  38. Transcription of chemically acetylated chromatin with homologous RNA polymerase B.
    Biosci Rep. 1984 Feb;4(2):155-63 PMID: 6713086
  39. Immunochemical detection of changes in chromatin subunits induced by histone H4 acetylation.
    EMBO J. 1982;1(8):939-44 PMID: 7188365
  40. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  41. ACETYLATION AND METHYLATION OF HISTONES AND THEIR POSSIBLE ROLE IN THE REGULATION OF RNA SYNTHESIS.
    Proc Natl Acad Sci U S A. 1964 May;51:786-94 PMID: 14172992
  42. In vitro transcription of RNA in nuclei, nucleoli and chromatin from physarum polycephalum.
    J Cell Sci. 1977 Aug;26:267-79 PMID: 562894
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.13.5405
Published
1984-07-11
Pages
5405-17
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318927
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/12.13.5405 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2