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PMID: 7001364 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

In vitro transcription of chromatin containing histones hyperacetylated in vivo.

Nucleic acids research ·Vol. 8 ·No. 18 ·1980-09-25 ·Pages 4201-19

Dobson ME, Ingram VM

Abstract

The culture of cells in the presence of sodium n-butyrate causes an accumulation of histones that are highly acetylated. When chromatin containing these histones was transcribed with E. coli RNA polymerase, an increase in the template activity compared to control chromatin was observed. Titration of chromatin with polymerase under both reinitiating and non-reinitiating conditions showed there was no increase in the number of regions available for transcription. Comparison of the kinetics for single and multiple rounds of transcription indicated that the rate of elongation was increased and probably the rate of reinitiation as well. Comparison of the size of transcripts from control and acetylated chromatin showed a small increase in the average size of transcripts from acetylated chromatin. When transcription was compared using partially purified HeLa polymerase, an increase was also seen. Studies under various ionic conditions showed that control chromatin required a higher salt concentration for optimum activity than did acetylated chromatin. In addition, at the optimum salt concentration for each chromatin, there was very little difference in the transcriptional activity using exogenous HeLa RNA polymerase.

MeSH Terms
Acetylation Chromatin/metabolism DNA-Directed RNA Polymerases/isolation & purification,metabolism Escherichia coli/enzymology HeLa Cells/metabolism Histones/metabolism Humans Templates, Genetic Transcription, Genetic
Chemicals
Chromatin Histones DNA-Directed RNA Polymerases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Dobson M E
Ingram V M
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50 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/8.18.4201
Published
1980-09-25
Pages
4201-19
Language
English
Region
England
NLM ID
0411011
PMCID
PMC324229
Subset
IM
Grants
NIADDK NIH HHS · AM13945 · United States
External Links
PubMed source DOI: 10.1093/nar/8.18.4201 PMC Full Text View journal details
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